| 施 莉,韦 炜,向 华.长链非编码RNA lnc-CDH11-3在胃癌组织中的表达及其干预效应分析[J].肿瘤学杂志,2018,24(8):790-794. |
| 长链非编码RNA lnc-CDH11-3在胃癌组织中的表达及其干预效应分析 |
| Expression of RNA lnc-CDH11-3 in Gastric Cancer and Its Clinicopathological Significance |
| 投稿时间：2017-06-13 |
| DOI：10.11735/j.issn.1671-170X.2018.08.B008 |
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| 中文关键词: 长链非编码RNA lnc-CDH11-3 胃肿瘤 增殖 侵袭 |
| 英文关键词:lncRNA lnc-CDH11-3 gastric cancer proliferation metastasis |
| 基金项目:浙江省科技厅课题（2014C33140）；浙江省金华市科技计划项目（2013-3-060） |
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| 中文摘要: |
| 摘 要：［目的］分析胃癌标本中长链非编码RNA lnc-CDH11-3的表达与临床病理特征的相关性，并在细胞水平上分析其对胃癌细胞的细胞活力、侵袭力及迁移能力的影响，探讨其在胃癌发生、发展中可能的调控及意义。［方法］ 收集46例胃癌组织及其癌旁正常组织标本，采用荧光实时定量PCR检测胃癌标本及癌旁正常胃黏膜组织中lnc-CDH11-3的表达量，并进一步分析临床病理特征与其表达水平的关系；利用慢病毒转染技术，建立lnc-CDH11-3表达量不同的胃癌稳定转染细胞株，运用CCK-8、Transwell跨膜实验检测细胞增殖活性、侵袭能力相关生物学功能；利用核浆分离及RT-PCR方法探究lnc-CDH11-3在细胞中的表达定位。［结果］ Lnc-CDH11-3在癌组织的表达量高于所对应的癌旁正常胃黏膜组织(P<0.05)。 Lnc-CDH11-3表达水平与肿瘤分化程度、区域淋巴结转移、TNM分期相关；而与年龄、性别、肿瘤大小、部位等其他因素无明显联系。稳定转染胃癌细胞株，成功构建lnc-CDH11-3低表达细胞株，CCK-8增殖实验结果显示下调lnc-CDH11-3可显著降低胃癌细胞株的增殖能力；Transwell跨膜实验显示沉默lnc-CDH11-3可显著降低胃癌细胞株的侵袭能力。用RT-PCR技术检测发现lnc-CDH11-3主要表达于细胞核中，仅少许表达于胞浆中。［结论］ Lnc-CDH11-3可作为促癌基因影响胃癌细胞增殖、侵袭等生物学特性，其表达量的变化与胃癌的发生发展密切相关。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the expression levels of lnc-CDH11-3 in gastric cancer(GC) and its relation to clinicopathological features of the tumor. ［Methods］ Forty-six specimens(cancer tissues and adjacent normal tissues) of GC were collected，the expression levels of lnc-CDH11-3 were detected by real-time RT-PCR，and its relationship with clinicopathological factors was analyzed. The gastric cancer BGC cells were transfected with lnc-CDH11-3 siRNA to silence the lnc-CDH11-3 expression. Cell proliferation and invasion were examined by CCK-8 assay and Transwell assay，respectively. Nucleocytoplasmic separation technology and RT-PCR were used to determine the intracellular localization of Inc-CDH11-3. ［Results］ The expression of lnc-CDH11-3 in GC tissue was upregulated in comparison with adjacent normal tissue(P<0.05). The expression of lnc-CDH11-3 was correlated with tumor differentiation，lymph nodes metastasis and TNM staging，but not correlated with age，gender，tumor size and tumor site. The CCK-8 assay showed that down-regulation of lnc-CDH11-3 significantly reduced the proliferation of BGC cells；and Transwell assay showed that silencing of Inc-CDH11-3 significantly reduced the invasive ability of BGC cells. RT-PCR found that lnc-CDH11-3 was mainly expressed in the nucleus，with only a little in the cytoplasm of GC cells. ［Conclusion］ lnc-CDH11-3 can promote proliferation and invasion of gastric cancer cells，and its expression levels are closely related to the clinicopathological features of gastric cancer. |
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