| 张 谢,谭 麟,毛琦淇.PTEN-Long通过PI3K-AKT途径对HepG2肝癌细胞的影响[J].肿瘤学杂志,2018,24(8):764-768. |
| PTEN-Long通过PI3K-AKT途径对HepG2肝癌细胞的影响 |
| Effects of PTEN-Long on PI3K-AKT Pathway in HepG2 Cells |
| 投稿时间：2017-10-27 |
| DOI：10.11735/j.issn.1671-170X.2018.08.B002 |
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| 中文关键词: 磷酸酶及张力蛋白同源基因剪接体 HepG2细胞系 细胞凋亡 磷酸肌醇3-激酶/蛋白激酶B |
| 英文关键词:PTEN-Long HepG2 lines apoptosis PI3K-AKT |
| 基金项目:浙江省公益技术应用研究计划项目（2017C35002）；宁波市重大民生项目（2013C51009）；浙江省医药卫生平台计划项目（2016DTA009） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨PTEN-Long对肝细胞癌HepG2细胞的影响及其脂质磷酸酶活性与PI3K-AKT信号通路之间的关系。［方法］ 细胞转染法转染PTEN-Long、PTEN-LongG302R和Vector于HepG2细胞。转染48h后，检测各组细胞增殖数，Western blot法检测各组细胞PTEN-Long、pAkt、pS6K表达变化，流式细胞法检测各组细胞细胞凋亡水平变化，细胞划痕法检测各组细胞迁移情况。［结果］ 细胞转染后，PTEN-Long组、PTEN-LongG320R组表达PTEN-Long蛋白量无显著性差异；与Vector、PTEN-LongG320R组相比，PTEN-Long组pAkt (Ser473)、pS6K (Thr389)表达量明显下降。从转染后48h开始，PTEN-Long组细胞增殖数较Vector 组、PTEN-LongG320R组明显减少，差异有统计学意义（P<0.05）；PTEN-Long组较Vector 组、PPTEN-LongG320R组凋亡明显增强（P<0.05）；划痕14h后，PTEN-Long组、PTEN-LongG320R组较Vector组的迁移细胞数明显减少（P<0.05）。［结论］ PTEN-long依赖于其脂质磷酸酶活性，通过PI3K-AKT通路调控肝癌HepG2细胞的增殖与凋亡。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of PTEN-Long on hepatocellular carcinoma HepG2 cells and the relationship between lipid phosphatase activity and PI3K-AKT signaling pathway. ［Methods ］ PTEN-Long，PTEN-LongG302R and Vector were transfected to HepG2 cells，respectively. After transfection for 48h，cell proliferation was detected in three groups，the expression of PTEN-Long，pAkt and pS6K was detected by Western blot，cell apoptosis was detected by flow cytometry，cell migration was detected by cell scratch assay. ［Results］ After transfection for 48 h，there was no significant difference in expression of PTEN-Long protein between PTEN-Long group and PTEN-LongG320R group. Compared with Vector and PTEN-LongG320R group，the expression of pAkt(Ser473) and pS6K(Thr389) in PTEN-Long group decreased significantly. From 48h after transfection，the cell proliferation in PTEN-Long group was significantly lower than that of PTEN-LongG320R group and Vector group(P<0.05). The apoptosis rate in PTEN-Long group was significantly higher than that in vector group and PPTEN-LongG320R group(P<0.05). The scratch assay showed that the number of migrating cells in PTEN-Long group and PTEN-LongG320R group was significantly lower than that in Vector group(P<0.05) . ［Conclusion］ PTEN-long regulates the proliferation and apoptosis of hepatocellular carcinoma HepG2 cells through PI3K-AKT pathway，which is lipid phosphatase activity-dependent. |
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