| 王晓燕.miR-203抑制bcl-w表达促进膀胱癌细胞凋亡[J].肿瘤学杂志,2018,24(6):597-600. |
| miR-203抑制bcl-w表达促进膀胱癌细胞凋亡 |
| miR-203 Promote Apoptosis of Bladder Cancer Cell by Expression-suppression of Bcl-w |
| 投稿时间：2017-09-14 |
| DOI：10.11735/j.issn.1671-170X.2018.06.B013 |
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| 中文关键词: miR-203 bcl-w 膀胱肿瘤 凋亡 |
| 英文关键词:miR-203 bcl-w bladder cancer apoptosis |
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| 中文摘要: |
| 摘 要：［目的］ 研究miR-203在膀胱癌组织中的表达，探讨其表达对膀胱癌细胞T24凋亡的影响。［方法］ 实时荧光定量PCR(qRT-PCR)检测23例膀胱癌组织和癌旁正常组织中miR-203表达情况，免疫印迹法检测bcl-w蛋白的表达情况，Pearson法分析miR-203表达与bcl-w蛋白表达相关性；转入miR-203-mimic建立高表达miR-203的T24细胞，流式细胞仪检测miR-203表达对T24细胞凋亡的影响。［结果］ miR-203在膀胱癌组织中的相对表达量为1.40±0.47，显著低于其在癌旁正常组织中的表达量（2.99±0.59）（P=0.0001）；miR-203在膀胱癌组织中的表达与bcl-w蛋白表达呈负相关（r=-0.952，P<0.001）；miR-203靶向抑制T24细胞中bcl-w蛋白的表达，转入miR-203-mimic 24h和72h后，T24细胞凋亡率分别为8.33%±0.58%和17.07%±0.76%，均显著高于对照组T24细胞的3.63%±0.32%和4.16%±0.28%（P=0.012，P<0.001）。［结论］ miR-203在膀胱癌组织中低表达，其可以通过靶向抑制bcl-w的表达促进膀胱癌细胞T24的凋亡。 |
| 英文摘要: |
| Abstract：［Objective］ To study the expression of miR-203 in bladder cancer，and to explore the effect of its expression on the apoptosis of T24 cells. ［Methods］ The expression of miR-203 in 23 bladder cancer tissue and adjacent normal tissue was detected by RT-qPCR，and the expression of bcl-w protein was detected by immunoblotting. The correlation of miR-203 and bcl-w was analyzed by Pearson. Transfer to miR-203-mimic to construct T24 cells with high expression of miR-203，and the effect of miR-203 expression on the apoptosis of T24 cells was detected by flow cytometry. ［Results］ The relative expression of miR-203 in bladder cancer was 1.40±0.47，which was significantly lower than that in adjacent normal tissues(2.99±0.59)(P=0.001). There was a negative correlation between miR-203 and bcl-w protein expression in bladder cancer tissue(r=-0.952，P<0.001). MiR-203 was used to inhibit the expression of bcl-w protein in T24 cells. After transfection into miR-203-mimic 24h and 72h，the apoptosis rate of T24 cells were 8.33%±0.58% and 17.07%±0.76%，respectively，which were significantly higher than those of control group 3.63%±0.32% and 4.16%±0.28%(P=0.012，P<0.001). ［Conclusion］ The expression of miR-203 is significantly downregulated in bladder cancer，and the expression of miR-203 can promote the apoptosis of T24 cells by repressing bcl-w expression. |
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