李 武,赵九洲,王志中.不同方法检测口咽鳞癌中高危型HPV的感染情况分析[J].肿瘤学杂志,2018,24(6):568-572.
不同方法检测口咽鳞癌中高危型HPV的感染情况分析
Detection of High-risk HPV Infection in Oropharyngeal Squamous Cell Carcinoma with Various Methods
投稿时间:2017-12-29  
DOI:10.11735/j.issn.1671-170X.2018.06.B008
中文关键词:  口咽鳞癌  HPV  p16免疫组化  二代杂交捕获  E6/E7
英文关键词:oropharyngeal squamous cell carcinoma  HPV  p16  hybrid capture-Ⅱ  E6/E7
基金项目:
作者单位
李 武 郑州大学第一附属医院 
赵九洲 郑州大学附属肿瘤医院 
王志中 郑州大学附属肿瘤医院 
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中文摘要:
      摘 要:[目的]分析不同方法检测口咽鳞癌中高危型HPV在河南地区人群中的感染情况。[方法] 采用二代杂交捕获、p16免疫组化、bDNA三种方法对89例口咽鳞癌石蜡包埋标本进行检测,并分析其HPV阳性率与患者临床病理参数的相关性及其临床意义。[结果] 二代杂交捕获、p16免疫组化、bDNA三种检测方法结果具有较好的一致性,HPV阳性率分别为27.0%,22.5%,16.9%。三种方法检出的HPV阳性结果与患者性别、年龄、原发灶部位、肿瘤分化程度、T分期等临床病理参数均无关(P>0.05)。经p16免疫组化法检测的结果与患者淋巴结转移(P=0.049)、TNM分期(P=0.026)存在相关性,而二代杂交捕获、bDNA结果与患者淋巴结转移、TNM分期(P>0.05)均无关。[结论] 在充分考虑临床实用性及可靠性的前提下,p16蛋白免疫组化法是最简单实用的HPV感染检测方法。
英文摘要:
      Abstract:[Objective] To investigate the infection of high-risk HPV in oropharyngeal squamous cell carcinoma by a variety of methods. [Methods] The hybrid capture-Ⅱ,p16 immunohistochemistry and bDNA methods were used to detect paraffin- embedded specimens from 89 patients with oropharyngeal squamous cell carcinoma. The correlation between the positive rate of HPV and clinicopathological parameters and its clinical significance were analyzed. [Results] The three detection methods showed a good consistency,and the positive rates of detection were 27%,22.5% and 16.9% respectively. The HPV results obtained from the three detection methods were not related to the gender,age,primary tumor location,tumor differentiation and T staging(P>0.05). The results of p16 immunohistochemistry were correlated with lymph node metastasis(P=0.049) and TNM staging(P=0.026),while the results of hybrid capture-Ⅱ and bDNA were not related to the lymph node metastasis and TNM staging(P>0.05). [Conclusion] Compared to other methods,p16 protein immunohistochemistry is the simplest and most effective method for high-risk HPV infection detection in patients with oropharyngeal squamous cell carcinoma.
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