| 钱露茜,尹 丽,顾佳佳.GINS2基因对鼻咽癌细胞5-8F生物学行为的影响[J].肿瘤学杂志,2018,24(6):536-541. |
| GINS2基因对鼻咽癌细胞5-8F生物学行为的影响 |
| Effects of GINS2 on Biological Behavior of Nasopharyngeal Carcinoma 5-8F Cell Line |
| 投稿时间：2017-10-19 |
| DOI：10.11735/j.issn.1671-170X.2018.06.B002 |
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| 中文关键词: 鼻咽肿瘤 GINS2基因 生长 增殖 凋亡 |
| 英文关键词:nasopharyngeal carcinoma GINS2 gene growth proliferation apoptosis |
| 基金项目:国家自然科学基金资助项目（81672989）；江苏省科技厅临床医学科技专项基金（BL2014091）；江苏省卫生与计划生育委员会青年基金（Q201501）；江苏省卫生厅青年医学人才基金（QNRC2016648） |
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| 中文摘要: |
| 摘 要：［目的］探讨GINS2基因对鼻咽癌5-8F细胞生物学行为的影响。［方法］将靶向GINS2基因的shRNA慢病毒载体感染5-8F细胞，利用荧光显微镜观察感染效率。实时荧光定量PCR检测GINS2 mRNA表达的改变；Western blot检测GINS2蛋白表达的改变；Celigo细胞成像分析、噻唑盐(MTT)法、平板克隆形成实验检测细胞的生长增殖能力；流式细胞术分析细胞凋亡率。［结果］ GINS2-shRNA慢病毒载体成功感染5-8F细胞，shGINS2组细胞中GINS2 mRNA和蛋白表达水平明显降低；Celigo细胞计数结果显示，shGINS2组细胞生长受抑制；MTT结果显示，shGINS2组细胞增殖受抑制；平板克隆形成实验显示，shGINS2组细胞克隆形成数减少；流式细胞术结果显示，shGINS2组细胞凋亡率增高。［结论］ 下调5-8F细胞GINS2的水平可抑制细胞生长增殖，诱导细胞凋亡。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effects of GINS2 on the biological behavior of nasopharygeal carcinoma(NPC) 5-8F cell line. ［Methods］The shRNA lentiviral vector targeting GINS2 was transfected into NPC 5-8F cells. The efficiency of transfection was observed under fluorescence microscope. Real-time quantitative PCR(qRT-PCR) and Western blot were used to detect the expression of GINS2 mRNA and protein respectively. Celigo image cytometry，MTT assay and colony formation assay were used to assess the viability and proliferation of 5-8F cells after transfection. Flow cytometry was used to detect the cell apoptosis. ［Results］ GINS2-shRNA was successfully transfected into 5-8F cells. qRT-PCR and Western Blot showed that the expressions of GINS2 mRNA and protein were significantly inhibited in shGINS2 group. Celigo image cytometry revealed that the growth ability of 5-8F cells in shGINS2 group was also inhibited. The MTT assay showed that the proliferation ability in shGINS2 group was suppressed. Colony formation assay showed that the colony number in shGINS2 group was reduced. Flow cytometry showed that the apoptosis rate increased in shGINS2 group. ［Conclusion］ The lentivirus-mediated GINS2 shRNA can inhibit the growth and proliferation，and promote the apoptosis of NPC 5-8F cells. |
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