| 邵 超,马永红,唐启胜.肿瘤相关成纤维细胞促进肝癌细胞HepG2生长及Wnt2基因的表达[J].肿瘤学杂志,2018,24(5):449-452. |
| 肿瘤相关成纤维细胞促进肝癌细胞HepG2生长及Wnt2基因的表达 |
| Carcinoma-Associated Fibroblasts Promote Growth of HepG2 Cells and Expression of Wnt2 |
| 投稿时间：2017-04-27 |
| DOI：10.11735/j.issn.1671-170X.2018.05.B008 |
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| 中文关键词: 肿瘤相关成纤维细胞 HepG2 Wnt2 |
| 英文关键词:carcinoma-associated fibroblasts HepG2 Wnt2 |
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| 摘要点击次数: 1949 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨肿瘤相关成纤维细胞(CAF)对肝癌细胞HepG2生长及Wnt2基因表达的影响。［方法］ 体外培养CAF并建立CAF与肝癌细胞HepG2相互作用体系，采用MTT法检测CAF 对肝癌细胞HepG2增殖能力的影响，qRT-PCR法与Western Blot检测CAF刺激导致的HepG2细胞中Wnt2基因与蛋白表达水平变化。［结果］ 通过MTT法检测CAF培养上清制备的条件培养基刺激HepG2细胞发现，与对照组相比，CAF上清刺激48h后能够明显促进HepG2细胞的增殖，96h OD490nm值达到最高（2.01±0.41，P<0.05）。qRT-PCR与Western Blot法检测CAF上清刺激后HepG2细胞中Wnt2表达发现，CAF上清能够在基因与蛋白水平增加Wnt2的表达，并明显高于对照组（P<0.05）。［结论］ CAF能够促进HepG2细胞的增殖，并且在基因与蛋白水平增加肝癌HepG2细胞Wnt2的表达。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of carcinoma-associated fibroblasts(CAFs) on the growth of HepG2 cells and the expression of Wnt2 gene. ［Methods］ CAFs were cultured in vitro and the interaction system of CAF and hepatoma HepG2 cell was established，then the effect of CAF on the proliferation of hepatoma HepG2 cells was detected by MTT assay. The expression of Wnt2 gene and protein in HepG2 cells induced by CAF stimulation was detected by qRT-PCR and Western-blotting，respectively. ［Results］ MTT assay showed that the proliferation of HepG2 cells was promoted by CAF culture supernatant stimulated for 48h，and reached the peak at 96h(2.01±0.41，P<0.05). The expression levels of Wnt2 gene and protein in HepG2 cells were increased significantly after stimulation by CAF supernatant(P<0.05). ［Conclusion］ CAF can promote the proliferation of HepG2 cells and increase the expression of Wnt2 gene and protein in HepG2 cells. |
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