王瑞娟,郑艳华,许 杨.miR-145在非小细胞肺癌中的表达及临床病理意义[J].肿瘤学杂志,2018,24(5):425-428.
miR-145在非小细胞肺癌中的表达及临床病理意义
Expression of miR-145 in Non-small Cell Lung Cancer and Its Clinicopathological Significance
投稿时间:2017-04-17  
DOI:10.11735/j.issn.1671-170X.2018.05.B003
中文关键词:  非小细胞肺癌  miR-145  组织  诊断  预后
英文关键词:non-small cell lung cancer  miR-145  tissue  diagnosis  prognosis
基金项目:首都医学发展科研基金重点支持项目(SF2011-5005-01)
作者单位
王瑞娟 中国人民解放军第306医院 
郑艳华 中国人民解放军第306医院 
许 杨 中国人民解放军第306医院 
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中文摘要:
      摘 要:[目的] 探讨miR-145在配对的非小细胞肺癌 (non-small cell lung cancer,NSCLC) 患者肿瘤组织和癌旁组织中的表达情况,分析其与临床病理因素和预后的关系。[方法] 应用实时定量聚合酶链反应(q-RT-PCR)检测miR-145在配对的NSCLC患者肿瘤组织和癌旁组织中的表达水平。[结果] miR-145在NSCLC患者肿瘤组织中的表达水平明显低于癌旁组织(P<0.001)。miR-145诊断NSCLC的ROC曲线下面积为0.895。miR-145在Ⅲ~Ⅳ期和中低分化的NSCLC患者肿瘤组织中的表达水平明显低于Ⅰ~Ⅱ期和高分化的肿瘤组织(P<0.05)。miR-145低表达组的总生存率明显低于miR-145高表达组(P<0.05)。[结论] miR-145在NSCLC组织中的表达下调,在NSCLC的发生中可能发挥抑癌基因的作用,可能是NSCLC的诊断及预后标志物之一。
英文摘要:
      Abstract:Abstract:[Objective] To explore the expression of miR-145 in non-small cell lung cancer(NSCLC) tissue and its adjacent tissue and its clinicopathological significance.[Methods] Expression level of miR-145 was detected in tumor tissue and its adjacent tissue by quantitative real-time PCR(qRT-PCR) from 62 patients with NSCLC. [Results] The expression of miR-145 in tumor tissue was significantly lower than that in the adjacent tissue(P<0.001). The area under the ROC curves(AUC) for miR-145 was 0.895. The expression of miR-145 was significantly lower in stage Ⅲ~Ⅳ and in moderate-poorly differentiated tumor tissue than that in stage Ⅰ~Ⅱ and in well-differentiated tumor tissue(P<0.05). The overall survival rate in patients with low expression miR-145 was significantly lower than those with high expression(P<0.05). [Conclusion] The expression of miR-145 is down-regulated in NSCLC. MiR-145 might function as anti-oncogene and be one of the potential biomarkers for the diagnosis and prognosis in patients with NSCLC.
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