| 迟笑怡,周 天,胡凯文.盐酸川芎嗪联合顺铂对小鼠Lewis肺癌细胞黏附能力的影响[J].肿瘤学杂志,2018,24(5):411-417. |
| 盐酸川芎嗪联合顺铂对小鼠Lewis肺癌细胞黏附能力的影响 |
| Effect of Tetramethylpyrazine Combined with Cisplatin on Migration and Invasion Ability of Mouse Lewis Lung Cancer Cells |
| 投稿时间：2017-06-08 |
| DOI：10.11735/j.issn.1671-170X.2018.05.B001 |
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| 中文关键词: Twist 肿瘤转移 盐酸川芎嗪 |
| 英文关键词:Twist cancer invasion tetramethylpyrazine |
| 基金项目:国家自然科学基金（81403250） |
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| 摘要点击次数: 1685 |
| 全文下载次数: 528 |
| 中文摘要: |
| 摘 要：［目的］ 探讨盐酸川芎嗪联合顺铂对小鼠Lewis肺癌细胞侵袭黏附能力的影响及其作用机制。［方法］ 小鼠Lewis肺癌细胞体外培养，细胞黏附侵袭实验检测细胞侵袭能力的改变，Western Blot检测各组细胞Twist、E-cadherin、N-cadherin蛋白表达的变化。［结果］ 经DDP与TMP干预36h后，与对照组相比，用药组细胞的侵袭能力呈剂量依赖性下降，且DDP联合TMP组与单独使用DDP组相比，抑制细胞侵袭的能力更为显著（P＜0.05，P＜0.01）。细胞黏附实验显示，与对照组（23.76±1.64）相比，单独应用顺铂（47.40±0.85）可显著性增高细胞黏附抑制率（P＜0.01），而单独应用TMP需药物浓度达到2000μg/ml时才可显著性增高黏附抑制率（P＜0.01），两药联合组黏附抑制率均高于单独使用DDP组，尤以高剂量组抑制率最为显著（P＜0.01）。与单独用药相比，DDP+TMP可降低Twist、N-cadherin蛋白，并上调E-cadherin蛋白的表达（P<0.01）。［结论］ 盐酸川芎嗪联合顺铂能抑制小鼠Lewis肺癌细胞的侵袭和转移，其机制可能与直接抑制肿瘤细胞侵袭，抑制相关基因蛋白Twist及N-cadherin，促进黏附分子E-cadherin蛋白表达有关。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of tetramethylpyrazine(TMP) and cisplatin(DDP) on migration and invasion ability of mouse Lewis lung cancer(LLC). ［Methods］ The cultured LLC cells were treated with DDP，TMP and DDP+TMP，respectively. The cell invasion ability was determined with cell adhesion assay，the expressions of Twist，E-cadherin and N-cadherin in LLC cells were detected by Western bloting. ［Results］ After 36h of treatment，compared with the control group，the invasion and migration of LLC cells in DDP，TMP and DDP+TMP groups were inhibited in a dose-dependent manner; and the effect of DDP+TMP was significantly stronger than that of TMP and DDP alone（P＜0.05，P＜0.01）. The adhesion assay showed that compared with control group（23.76±1.64），the inhibition rate of adhesion in DDP group（47.40±0.85）and high dose-TMP group(65.727.78)was dramatically increased（P＜0.01），and that in DDP+TMP group，especially in DDP+ high dose-TMP group was significantly higher than that in DDP group（P＜0.01）. Western blot showed that TMP and DDP reduced the expression of Twist，N-cadherin and increase the expression of E-cadheri（P<0.01）. ［Conclusion］ Tetramethylpyrazine and DDP can inhibit invasion and metastasis of LLC cells，which may be associated with inhibition of Twist，N-cadherin gene expression and increase of the adhesion molecule E-cadherin expression. |
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