邓 野,蔡 君,王 炫.RSK4对结直肠癌细胞增殖的影响[J].肿瘤学杂志,2018,24(4):341-345. |
RSK4对结直肠癌细胞增殖的影响 |
Effect of RSK4 on Proliferation of Colorectal Cancer Cells |
投稿时间:2017-01-10 |
DOI:10.11735/j.issn.1671-170X.2018.04.B010 |
|
|
中文关键词: RSK4 结直肠肿瘤 抑癌基因 |
英文关键词:RSK4 colorectal cancer tumor suppressor genes |
基金项目:2015年湖北省卫计委一般项目(WJ2015MB212) |
|
摘要点击次数: 1597 |
全文下载次数: 409 |
中文摘要: |
摘 要:[目的] 探讨RSK4(p90 ribosomalprotein S6 kinase4)在结直肠癌细胞的表达情况及其生物学作用。[方法] 挑选结直肠癌细胞株SW480和HCT116,用Lipofectamin 2000技术将RSK4基因转染至细胞核内,建立结直肠癌RSK4基因高表达细胞系,用RT-PCR及WB证实了RSK4在结直肠癌细胞SW480和HCT116转染成功,采用MTT、流式细胞仪技术检测转染RSK4后对结直肠癌细胞增殖的影响。[结果] RSK4在结直肠癌细胞呈低表达,转染成功后对结直肠癌细胞有明显的抑制作用,并使S 期细胞所占的比例下降,G0 / G1期比例上升(P<0.05)。[结论] RSK4基因在结直肠癌细胞株中呈低表达,转染RSK4后可抑制肿瘤细胞生长,有可能为一个潜在的抑癌基因。 |
英文摘要: |
Abstract:[Objective] To investigate the effects of RSK4(P90 Ribosomal Protein S6) in colorectal cancer cells. [Methods] RSK4 gene was transfected into colorectal cancer SW480 and HCT116 cells by Lipofectamin 2000. The expression of RSK4 in wild and transtected SW480 and HCT116 cells was confirmed by RT-PCR and Western blot. Cell proliferation was detected by MTT and flow cytometry. [Results] RSK4 was lowly expressed in wild SW480 and HCT116 cells,while RSK4 was over-expressed in transfected cells. MTT assay showed that the proliferation was significantly inhibited in RSK4-transfected colorectal cancer cells. Flow cytometry revealed that S phase cells decreased and G0/1 cells increased significantly in transfected colorectal cancer cells(P<0.05).[Conclusion] Low expression of RSK4 gene is detected in colorectal cancer cells,and transfection of RSK4 inhibits cancer cell proliferation,which indicates that RSK4 may be a tumor suppressor gene in colorectal cancer. |
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |