| 邓 野,蔡 君,王 炫.RSK4对结直肠癌细胞增殖的影响[J].肿瘤学杂志,2018,24(4):341-345. |
| RSK4对结直肠癌细胞增殖的影响 |
| Effect of RSK4 on Proliferation of Colorectal Cancer Cells |
| 投稿时间：2017-01-10 |
| DOI：10.11735/j.issn.1671-170X.2018.04.B010 |
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| 中文关键词: RSK4 结直肠肿瘤 抑癌基因 |
| 英文关键词:RSK4 colorectal cancer tumor suppressor genes |
| 基金项目:2015年湖北省卫计委一般项目(WJ2015MB212) |
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| 中文摘要: |
| 摘 要：［目的］ 探讨RSK4（p90 ribosomalprotein S6 kinase4）在结直肠癌细胞的表达情况及其生物学作用。［方法］ 挑选结直肠癌细胞株SW480和HCT116，用Lipofectamin 2000技术将RSK4基因转染至细胞核内，建立结直肠癌RSK4基因高表达细胞系，用RT-PCR及WB证实了RSK4在结直肠癌细胞SW480和HCT116转染成功，采用MTT、流式细胞仪技术检测转染RSK4后对结直肠癌细胞增殖的影响。［结果］ RSK4在结直肠癌细胞呈低表达，转染成功后对结直肠癌细胞有明显的抑制作用，并使S 期细胞所占的比例下降，G0 / G1期比例上升（P<0.05）。［结论］ RSK4基因在结直肠癌细胞株中呈低表达，转染RSK4后可抑制肿瘤细胞生长，有可能为一个潜在的抑癌基因。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effects of RSK4(P90 Ribosomal Protein S6) in colorectal cancer cells. ［Methods］ RSK4 gene was transfected into colorectal cancer SW480 and HCT116 cells by Lipofectamin 2000. The expression of RSK4 in wild and transtected SW480 and HCT116 cells was confirmed by RT-PCR and Western blot. Cell proliferation was detected by MTT and flow cytometry. ［Results］ RSK4 was lowly expressed in wild SW480 and HCT116 cells，while RSK4 was over-expressed in transfected cells. MTT assay showed that the proliferation was significantly inhibited in RSK4-transfected colorectal cancer cells. Flow cytometry revealed that S phase cells decreased and G0/1 cells increased significantly in transfected colorectal cancer cells（P<0.05）.［Conclusion］ Low expression of RSK4 gene is detected in colorectal cancer cells，and transfection of RSK4 inhibits cancer cell proliferation，which indicates that RSK4 may be a tumor suppressor gene in colorectal cancer. |
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