董锐增,张则伟,周一鸣.重楼皂甙Ⅰ对胃癌肿瘤相关成纤维细胞的作用[J].肿瘤学杂志,2018,24(4):336-340. |
重楼皂甙Ⅰ对胃癌肿瘤相关成纤维细胞的作用 |
The Effect of Polyphyllin Ⅰon Gastric Cancer-associated Fibroblasts |
投稿时间:2017-04-20 |
DOI:10.11735/j.issn.1671-170X.2018.04.B009 |
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中文关键词: 胃肿瘤 重楼皂甙Ⅰ 肿瘤相关成纤维细胞 |
英文关键词:stomach neoplasms polyphyllin Ⅰ cancer-associated fibroblasts |
基金项目:浙江省中医药管理局基金资助项目(2013ZA019) |
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中文摘要: |
摘 要:[目的] 探讨重楼皂甙Ⅰ对胃癌肿瘤相关成纤维细胞(cancer-associated fibroblasts,CAFs)的作用。[方法] 以体外原代培养的胃癌CAFs为研究对象,MTT法检测重楼皂甙Ⅰ对CAFs细胞的增殖抑制作用,Western Blot法检测CAFs细胞FAP、SPARC、SDF-1、TNC和HGF蛋白表达改变。[结果] 不同浓度重楼皂甙Ⅰ能有效抑制CAFs细胞的增殖,且呈浓度依赖性。2μg/ml重楼皂甙Ⅰ作用CAFs细胞24h后,FAP、SDF-1和HGF蛋白表达降低,与对照组相比差异均有统计学意义(P<0.05)。SPARC和TNC蛋白表达改变与对照组相比差异无统计学意义(P>0.05)。[结论] 重楼皂甙Ⅰ能抑制胃癌CAFs细胞的体外增殖,通过下调FAP、SDF-1和HGF蛋白表达影响CAFs功能。 |
英文摘要: |
Abstract:[Objective] To explore the effect of polyphyllin Ⅰ(PPⅠ) on gastric cancer-associated fibroblasts. [Methods] The primary gastric CAFs were obtained by tissue culture. The effects of PPⅠ on cell proliferation were detected by MTT method. The expressions of FAP,SPARC,SDF-1,TNC and HGF were detected by Western Blot. [Results] The growth of CAFs cells was inhibited by PPⅠ in dose-dependent manner. Compared with control groups,the expressions of FAP,SDF-1 and HGF protein decreased significantly(P<0.05)after treated with 2μg/ml PPⅠ at 24h,while the expressions of SPARC and TNC protein were not statistically significant(P>0.05). [Conclusion] PPⅠ can inhibit the cell proliferation of gastric CAFs and affect the function of gastric CAFs by down-regulating the expression of FAP,SDF-1 and HGF protein. |
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