| 赖 彦,邓述恺.谷氨酰胺对肺腺癌A549细胞增殖、迁移及TNF-α和NF-κB表达的影响[J].肿瘤学杂志,2018,24(3):213-217. |
| 谷氨酰胺对肺腺癌A549细胞增殖、迁移及TNF-α和NF-κB表达的影响 |
| Effects of Glutamine on Proliferation，Migration and Expression of TNF-α and NF-κB in Lung Adenocarcinoma A549 Cells |
| 投稿时间：2017-06-06 |
| DOI：10.11735/j.issn.1671-170X.2018.03.B007 |
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| 中文关键词: 谷氨酰胺 A549细胞 Transwell TNF-α NF-κB |
| 英文关键词:glutamine A549 cells transwell TNF-α NF-κB |
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| 中文摘要: |
| 摘 要：［目的］ 探讨谷氨酰胺（glutamine，Gln）对肺腺癌A549细胞增殖、迁移及TNF-α、NF-κB表达的影响。［方法］ 体外培养肺腺癌A549细胞株，CCK-8法检测不同浓度Gln（2、4、8、16、32、64 mmol/L）对A549细胞的增殖影响，筛选适宜浓度。Transwell 实验检测细胞迁移能力，ELISA法和Western Bolt法分别检测TNF-α、NF-κB p65蛋白表达。［结果］ （1） Gln对A549细胞的作用与干预浓度有关，缺乏Gln时细胞生长受限；低浓度Gln（2、4、8mmol/L）对细胞增殖无明显抑制作用；高浓度Gln（16、32、64mmol/L）作用下抑制作用明显（P<0.01）并呈时间依赖性，且32mmol/L和64mmol/L两组作用无明显差异（P>0.05）。（2）与对照组相比，Gln组明显降低A549细胞迁移数；高浓度Gln组明显降低TNF-α、NF-κB p65蛋白的表达（P<0.01），但32mmol/L和64mmol/L组之间无统计学差异（TNF-α分别为14.671±0.842pg/ml、14.867±1.21pg/ml，NF-κB p65分别为0.548±0.042、0.474±0.077）（P>0.05）。［结论］ Gln对肺腺癌A549细胞的作用与浓度有关，32mmol/L可能为最适抑制浓度。Gln可能通过下调TNF-α和NF-κB p65蛋白表达并抑制A549细胞的迁移发挥抗肺腺癌作用。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effects of glutamine on proliferation，migration and expression of TNF-α and NF-κB in human lung adenocarcinoma A549 cells. ［Methods］ The cultured lung adenocarcinoma A549 cells were treated with glutamine(0，2，4，8，16，32，64mmol/L）. The cell growth inhibition rate was measured with CCK-8 method；the migration ability of A549 cells was determined by Transwell assay；the expression of TNF-α was detected by ELISA method and NF-κB p65 protein was detected by Western Blot. ［Results］ The inhibition rates of A549 cells in glutamine 16，32 and 64mmol/L groups were significantly higher than those in control group and glutamine 2，4，8mmol/L groups（P<0.01），while there was no significant difference between 32mmol/L and 64mmol/L groups（P>0.05）. Compared with the control group，the migration ability of A549 cells in glutamine 32mmol/L group was significantly reduced；and the expressions of TNF-α and NF-κB p65 protein in in glutamine 16，32 and 64mmol/L groups were significantly decreased（P<0.01），but there was no significant difference between 32mmol/L and 64mmol/L groups（P>0.05）. ［Conclusion］ The effect of glutamine on lung adenocarcinoma A549 cells is concentration-dependent，and the optimal inhibitory concentration is 32mmol/L. The effect of glutamine on lung adenocarcinoma cells may be related to down-regulation of TNF-α and NF-κB p65 in A549 cells. |
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