| 王 丽,周淑亭,黄玉红.膜联蛋白A7对肿瘤细胞黏附分子表达及生物学行为的影响[J].肿瘤学杂志,2018,24(1):31-36. |
| 膜联蛋白A7对肿瘤细胞黏附分子表达及生物学行为的影响 |
| Effect of Annexin A7 on Expression of Adhesion Molecules and Biological Behavior in Mouse Hepatocarcinoma Hca-P Cells |
| 投稿时间：2017-02-21 |
| DOI：10.11735/j.issn.1671-170X.2018.01.B007 |
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| 中文关键词: 肝癌 Annexin A7 FAK Src E-cadherin 淋巴结粘附 细胞侵袭 |
| 英文关键词:liver cancer AnnexinA7 FAK Src E-cadherin lymph node adhesion cell invasion |
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| 中文摘要: |
| 摘 要：［目的］ 研究Annexin A7基因对肿瘤细胞黏附分子表达及其生物学行为的影响。［方法］ 采用shRNA干扰技术稳定转染小鼠Hca-P细胞，获得Annexin A7下调表达及Hca-P无关序列细胞株，采用Western Blot、qRT-PCR分别检测Annexin A7对黏附相关分子FAK、Src、E-cadherin等基因及蛋白表达水平的影响，应用淋巴结粘附及Transwell侵袭实验检测Annexin A7下调对Hca-P细胞淋巴结粘附及侵袭能力的干扰。［结果］ Annexin A7基因表达下调组细胞的FAK和Src基因及蛋白表达水平较A7无关序列组分别上升1.81倍、1.80倍及1.58倍、1.59倍（P<0.05），E-cadherin基因及蛋白表达水平则分别下降54.11%和54.27%，而上述分子基因与蛋白表达水平在未处理Hca-P组与A7无关序列组间无统计学差异（P>0.05），且在Annexin A7下调后Hca-P细胞的淋巴结粘附及侵袭能力上升，ShRNA-AnnexinA7组淋巴结黏附细胞数(1074±162.8)显著性多于A7无关序列组（234.7±40.08）及Hca-P组（220.3±39.53）（P<0.05），ShRNA- A7细胞组穿过小室的细胞数目（294.8±64.40）明显多于A7无关序列细胞组（79±28.04）及Hca-P细胞组（57.60±25.62）（P<0.05），而Hca-P与无关序列细胞组之间则无统计学差异（P>0.05）。［结论］ Annexin A7可通过影响黏附相关分子FAK、Src、E-cadherin等基因与蛋白表达水平从而改变Hca-P细胞生物学行为。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of Annexin A7 on the expression of adhesion molecules and biological behavior in mouse hepatocarcinoma cells. ［Methods］ Mouse heparocarcinoma Hca-P cells were transfected with Annexin A7 shRNA(AnnexinA7-shRNA group) or unrelated shRNA(blank control group). The expressions of Annexin A7 and the adhesion molecules FAK，Src，E-cadherin in Hca-P cells were detected by Western Blot and qRT PCR. Transwell assay and lymph node adhesion testing were used to analyze the invasion and adhesion ability of Hca-P cells after Annexin A7 shRNA interference. ［Results］ Compared with the blank control group，the expression of Annexin A7 was reduced in AnnexinA7-shRNA group(P<0.05); the mRNA and protein expression levels of FAK and Src were increased by 1.81，1.80 times and 1.58，1.59 times(P<0.05)，respectively，while the mRNA and protein expression levels of E-cadherin were decreased by 54.11% and 54.27%，respectively(P<0.05). Meanwhile，the invasion and adhesion ability in AnnexinA7- shRNA group was significantly higher than wild Hca–P cells and blank control group，the number of lymph node cells in ShRNA-AnnexinA7 group(1074 ± 162.8) was significantly higher than that in blank control group(234.7 ± 40.08) and wild Hca-P cell group(220.3±39.53)(P<0.05)，the number of migrant cells in ShRNA-AnnexinA7 group（294.8±64.40）was significantly higher than that in blank control group(79±28.04） and wild Hca-P cell group（57.60±25.62）(P<0.05)，while there was no significant difference between wild Hca-P cell and blank control groups(P>0.05). ［Conclusion］ Annexin A7 may change biological behavior of hepatocarcinoma Hca-P cells by influencing the gene and protein expression of adhesion molecules FAK，Src and E-cadherin. |
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