| 林昌海,黄露迷,冀晓辉.非小细胞肺癌患者外周血Treg和Th17水平及意义[J].肿瘤学杂志,2018,24(1):27-30. |
| 非小细胞肺癌患者外周血Treg和Th17水平及意义 |
| Treg and Th17 Cells in Peripheral Blood of Patients with Non-small Cell Lung Cancer and Their Signification |
| 投稿时间：2017-03-07 |
| DOI：10.11735/j.issn.1671-170X.2018.01.B006 |
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| 中文关键词: Treg细胞 Th17细胞 非小细胞肺癌 肿瘤免疫 |
| 英文关键词:regulatory T cells Th17 cells non-small cell lung cancer tumor immunity |
| 基金项目:重庆市卫计委项目（2010-2-305，2011-2-350）；重庆市自然科学基金（#2013jcyjA10125） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨非小细胞肺癌(NSCLC)患者外周血中Treg和Th17细胞的变化及其意义。［方法］ 收集40例NSCLC患者和20名健康对照的外周血样本，采用流式细胞术检测外周血中Treg和Th17细胞占CD4+T细胞的比例，酶联免疫吸附实验法检测TGF-β和IL-17水平。［结果］ 肺癌患者外周血中Treg细胞和Th17细胞比例均显著性高于健康对照组(分别为6.65%±3.70% vs 4.06%±1.23%和1.20%±1.19% vs 0.44%±0.13%，P<0.05)。外周血Treg和Th17细胞表达与NSCLC患者分期等无相关性(P>0．05)。肺癌患者外周血TGF-β和IL-17水平明显高于对照组(分别为25.05±3.19 vs 15.96±3.65 和4.36±2.79 vs 2.01±1.83，P<0.05)。［结论］ NSCLC患者外周血中Treg和Th17细胞以及相关细胞因子均高表达，Treg细胞以及Th17细胞参与了肺癌的发生和发展。 |
| 英文摘要: |
| Abstract：［Objective］ To determine the levels of Treg and Th17 cells in peripheral blood of patients with non-small cell lung cancer(NSCLC) and their significance．［Methods］ The peripheral blood samples of 40 NSCLC patients and 20 health subjects were collected. The Treg and Th17 cells in peripheral blood were detected by flow cytometry，the serum TGF-β and IL-17 levels were measured by ELISA．The relationship between the expression of Treg and Th17 cells and clinicopathological features of NSCLC patients was analyzed．［Results］ The expression of Treg and Th17 cells in peripheral blood of NSCLC patients were significantly higher than that in healthy controls(6.65%±3.70% vs 4.06%±1.23% and 1.20%±1.19% vs 0.44%±0.13%，both P<0.05)．However the levels of Treg and Th17 were not correlated with the stage of NSCLC(P>0．05)．The serum TGF-β and IL-17 levels in NSCLC patients were significantly higher than those in controls(25.05±3.19 vs 15.96±3.65 and 4.36±2.79 vs 2.01±1.83，both P<0.05). ［Conclusion］ Treg and Th17 cells and their associated cytokines TGF-β and IL-17 are highly expressed in peripheral blood of NSCLC patients. |
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