魏丽娟,曹子佩,朱伟智.扁柏醇体外对肾癌细胞的影响[J].肿瘤学杂志,2017,23(12):1098-1101.
扁柏醇体外对肾癌细胞的影响
The Effect of Hinokitol on Renal Carcinoma Cell in vitro
投稿时间:2017-01-04  
DOI:10.11735/j.issn.1671-170X.2017.12.B010
中文关键词:  扁柏醇  肾癌  细胞周期  凋亡  SPAK/JNK
英文关键词:hinokitol  renal carcinoma  cell cycle  apoptosis  SPAK/JNK
基金项目:
作者单位
魏丽娟 宁波市泌尿肾病医院 
曹子佩 宁波市泌尿肾病医院 
朱伟智 宁波市泌尿肾病医院 
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中文摘要:
      摘 要:[目的] 探讨扁柏醇对肾癌细胞生长、细胞周期和凋亡的影响及其可能作用机制。 [方法] 以人肾癌786-0细胞为模型,应用MTT法检测扁柏醇对786-0细胞生长抑制,应用流式细胞术检测经扁柏醇处理后786-0细胞周期变化,应用AnnexinV/PI双染法检测扁柏醇诱导肾癌细胞凋亡的发生,用western blot方法分析PARP、JNK蛋白表达和活性的变化。[结果] 扁柏醇对786-0细胞的生长有明显抑制作用,呈浓度依赖性和时间依赖性;随着扁柏醇浓度的增加,处于S期的细胞逐渐增加,而G2/M期的细胞逐渐减少;扁柏醇作用后凋亡细胞也明显增加,并伴随着cleaved-PARP的表达增加;p-JNK蛋白含量随着扁柏醇的浓度增加而升高,而JNK蛋白含量无明显变化。[结论] 扁柏醇能明显抑制人肾癌786-0细胞的生长,可能与扁柏醇将细胞阻滞于S期和通过激活SPAK/JNK信号通路诱导细胞凋亡有关。
英文摘要:
      Abstract:[Objective] To explore the effect of hinokitol on growth,cell cycle and apoptosis of renal carcinoma cell and its possible mechanism. [Methods] Experiments were performed on human renal carcinoma 786-0 cell. The inhibitory effect of hinokitol on 786-0 cell was evaluated by MTT test. Flow cytometry was used to measure the cell cycle. Apoptosis induction was assessed by Annexin V-FITC/PI double staining assay. The expression and activity of PARP and JNK was detected by Western blot assay. [Results] Hinokitol could inhibit the growth of 786-0 cell in a time and dose dependent manner. With the concentration of hinokitol increasing,S percentage was dramatically increased while G2/M percentage was significantly decreased.786-0 cell treated with hinokitol displayed much higher apoptosis than the control group accompanied by increased cleaved-PARP. The expression level of p-JNK was up-regulated and the level of JNK was unchangeable after treatment with evodiamine. [Conclusion] Hinokitol can inhibit the growth of 786-0 cell through cell cycle arrest and apoptosis by activating SPAK/JNK signaling.
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