| 中文关键词: 扁柏醇 肾癌 细胞周期 凋亡 SPAK/JNK |
| 英文关键词:hinokitol renal carcinoma cell cycle apoptosis SPAK/JNK |
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| 中文摘要: |
| 摘 要：［目的］ 探讨扁柏醇对肾癌细胞生长、细胞周期和凋亡的影响及其可能作用机制。 ［方法］ 以人肾癌786-0细胞为模型，应用MTT法检测扁柏醇对786-0细胞生长抑制，应用流式细胞术检测经扁柏醇处理后786-0细胞周期变化，应用AnnexinV/PI双染法检测扁柏醇诱导肾癌细胞凋亡的发生，用western blot方法分析PARP、JNK蛋白表达和活性的变化。［结果］ 扁柏醇对786-0细胞的生长有明显抑制作用，呈浓度依赖性和时间依赖性；随着扁柏醇浓度的增加，处于S期的细胞逐渐增加，而G2/M期的细胞逐渐减少；扁柏醇作用后凋亡细胞也明显增加，并伴随着cleaved-PARP的表达增加；p-JNK蛋白含量随着扁柏醇的浓度增加而升高，而JNK蛋白含量无明显变化。［结论］ 扁柏醇能明显抑制人肾癌786-0细胞的生长，可能与扁柏醇将细胞阻滞于S期和通过激活SPAK/JNK信号通路诱导细胞凋亡有关。 |
| 英文摘要: |
| Abstract：［Objective］ To explore the effect of hinokitol on growth，cell cycle and apoptosis of renal carcinoma cell and its possible mechanism. ［Methods］ Experiments were performed on human renal carcinoma 786-0 cell. The inhibitory effect of hinokitol on 786-0 cell was evaluated by MTT test. Flow cytometry was used to measure the cell cycle. Apoptosis induction was assessed by Annexin V-FITC/PI double staining assay. The expression and activity of PARP and JNK was detected by Western blot assay. ［Results］ Hinokitol could inhibit the growth of 786-0 cell in a time and dose dependent manner. With the concentration of hinokitol increasing，S percentage was dramatically increased while G2/M percentage was significantly decreased.786-0 cell treated with hinokitol displayed much higher apoptosis than the control group accompanied by increased cleaved-PARP. The expression level of p-JNK was up-regulated and the level of JNK was unchangeable after treatment with evodiamine. ［Conclusion］ Hinokitol can inhibit the growth of 786-0 cell through cell cycle arrest and apoptosis by activating SPAK/JNK signaling. |
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