| 张建东,贺利民,马 磊.miR-21、PDCD4表达对Ⅱ期食管鳞癌术后患者生存期的影响[J].肿瘤学杂志,2017,23(12):1047-1054. |
| miR-21、PDCD4表达对Ⅱ期食管鳞癌术后患者生存期的影响 |
| The Effect of MiR-21 and PDCD4 Ｅxpression on the Survival of Patients with Stage Ⅱ Esophageal Carcinoma Ａfter Operation |
| 投稿时间：2017-06-05 |
| DOI：10.11735/j.issn.1671-170X.2017.12.B001 |
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| 中文关键词: 食管鳞癌 miR-21 PDCD4 生存期 预后 |
| 英文关键词:esophageal squamous cell carcinoma miR-21 PDCD4 survival prognosis |
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| 中文摘要: |
| 摘 要：［目的］ 探讨微小RNA 21（microRNA-21，miR-21）和细胞程序性死亡蛋白（programmed cell death protein 4，PDCD4）在Ⅱ期食管鳞癌术后患者癌组织中表达，分析其与临床病理特征的关系及对预后的影响。［方法］ 收集胸中段Ⅱ期食管癌术后患者标本97例，将样本组织脱蜡提取总RNA；应用实时免疫荧光定量聚合酶链反应（RT-PCR）法检测所有标本癌组织、癌旁组织miR-21的表达情况；免疫组化法检测测癌组织和癌旁组织PDCD4蛋白表达水平。 ［结果］ miR-21在Ⅱ期食管鳞癌术后患者癌组织中的表达较癌旁组织升高（t=12.06，P＜0.01），PDCD4在癌组织中的表达较癌旁组织降低（t=8.36，P＜0.01）。miR-21和PDCD4在癌组织中的表达呈负相关（r=-0.41，P＜0.01），在癌旁组织中的表达呈正相关（r=0.51，P＜0.01）。癌组织中miR-21的表达与食管鳞癌的浸润深度、淋巴结转移、内镜下病变长度呈正相关（r=0.23，P=0.02；r=0.22，P=0.03；r=0.38，P＜0.01）、与分化程度呈负相关（r=-0.60，P＜0.01），PDCD4蛋白的表达与食管鳞癌的浸润深度、淋巴结转移、内镜下病变长度负相关（r=-0.21，P=0.04；r=-0.24，P=0.01；r=-0.37，P＜0.01）、与分化程度呈正相关（r=0.53，P＜0.01），二者与年龄、性别、位置不相关（P＞0.05）。COX回归模型发现Ⅱ期食管鳞癌患者肿瘤浸润深度、淋巴结转移、分化程度、内镜下病变长度、miR-21表达、PDCD4表达均为影响患者PFS的因素（?字2=10.36，P=0.01；?字2=7.89，P＜0.01；?字2=6.48，P=0.02；?字2=5.32，P=0.03；?字2=9.56，P=0.01；?字2=5.40，P=0.02）。miR-21对于术后3年PFS、OS预测曲线下面积分别为73.5%、81.0%（P<0.01）；PDCD4对于术后3年PFS、OS预测曲线下面积分别为77.3%、73.2%（P<0.01）。miR-21、PDCD4高、低表达组PFS差异有统计学意义（?字2=19.6，P＜0.01；?字2=11.45，P＜0.01），OS差异也有统计学意义（?字2=27.84，P＜0.01；?字2=21.35，P＜0.01）。［结论］Ⅱ期食管鳞癌术后患者癌组织与癌旁正常组织中miR-21、PDCD4蛋白表达存在明显差异。高表达miR-21提示预后较差，而高表达PDCD4提示预后较好。 |
| 英文摘要: |
| Abstract：［Objective］ To study the expression of RNA-21 (microR-21 or miR-21) and PDCD4 (programmed cell death protein 4) in patients with stage Ⅱ esophageal squamous cell carcinoma after operation. To analyze the correlations among microR-21，PDCD4 and clinicopathological features as well as the effect of their expression on prognosis. ［Methods］ A total of 97 samples were collected from the mid-thoracic portion of patients with stage Ⅱ esophageal squamous cell carcinoma. Total RNA of these sample tissues was extracted by dewaxing. The expression level of miR-21 in all sample cancer tissues and adjacent tissues was detected by RT-PCR (real-time immunofluorescence quantitative polymerase chain reaction). And the expression level of PDCD4 in cancer tissues and adjacent normal tissues was detected by immunohistochemistry. ［Results］ The expression of miR-21 in cancer tissues was higher than that in adjacent normal tissues (t=12.06，P＜0.01)，and the expression of PDCD4 protein in cancer tissues was lower than that in adjacent normal tissues (t=8.36，P＜0.01). The correlation analysis revealed a negative correlation (r=-0.41，P＜0.01) of the expression of miR-21 and PDCD4 in cancer tissues and a positive correlation (r=0.51，P＜0.01 )of their expression in adjacent normal tissues. The expression of miR-21 in cancer tissues was positively correlated with the depth of tumor invasion，lymph node metastasis and endoscopic lesion length (r=0.23，P=0.02；r=0.22；P=0.03；r=0.38；P＜0.01)，and negatively correlated with the differentiation degree(r=-0.60，P＜0.01). The expression of PDCD4 protein in cancer tissues was negatively correlated with the depth of tumor invasion，lymph node metastasis and endoscopic lesion length(r=-0.21，P=0.04；r=-0.24，P=0.01；r=-0.37，P＜0.01)，and positively correlated with the differentiation (r=0.53，P＜0.01). The expression of both was not correlated with age，gender and location(P>0.05). A COX regression model was established to reveal that the depth of tumor invasion，lymph node metastasis，differentiation degree，endoscopic lesion length，expression of microR-21 and expression of PDCD4 were all factors that affect PFS of patients with stage Ⅱ esophageal squamous cell carcinoma after operation(X2=10.36，P=0.01；X2=7.89，P＜0.01；X2=6.48，P=0.02；X2=5.32，P=0.03，X2=9.56，P=0.01；X2=5.40，P=0.02，respectively). For miR-21，the area under the 3-year PFS and OS prediction curve was 73.5% and 81.0%，respectively(all P＜0.01). For PDCD4，the area under the 3-year PFS and OS prediction curve was 77.3% and 73.2%，respectively expression. According to the cutoff values，different expression results of miR-21 and PDCD4 were divided into high and low expression groups. Kaplan-Meier was used to find that different expression of miR-21 and PDCD4 were significantly different in PFS(the results were X2=19.6，P＜0.01；X2=11.45，P＜0.01) and in OS(X2=27.84，P＜0.01；X2=21.35，P＜0.01). ［Conclusion］There were significant differences in the expression of miR-21 and PDCD4 protein between cancerous tissues and adjacent normal tissues in patients with stage Ⅱ esophageal squamous cell carcinoma after operation. Highly expressed miR-21 suggested poor prognosis，while the high expression of PDCD4 protein suggested better prognosis. |
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