| 马 欢,刘 敏,陈雪祺.131I-c(RGD)2环肽及其联合基因治疗对荷神经胶质瘤裸鼠模型肿瘤抑制作用[J].肿瘤学杂志,2017,23(10):837-841. |
| 131I-c(RGD)2环肽及其联合基因治疗对荷神经胶质瘤裸鼠模型肿瘤抑制作用 |
| 131I-c(RGD)2 Combined Lentiviral Vector E8-codA-GFP in Treatment of Human Glioblastoma in Nude Mice |
| 投稿时间：2016-10-21 |
| DOI：10.11735/j.issn.1671-170X.2017.10.B001 |
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| 中文关键词: 放射敏感性启动子 CD/5-FC自杀系统 基因治疗 131I-c(RGD)2 |
| 英文关键词:radiation-responsive promoter CD/5-FC suicide system gene therapy 131I-c(RGD)2 |
| 基金项目:北京市自然科学基金资助项目（放射敏感性基因联合放射免疫治疗对膀胱癌的治疗作用与毒性研究）（7112129） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨131I标记的c(RGD)2对荷神经胶质瘤裸鼠模型的治疗作用，并与放射敏感性启动子E8和CD/5-FC自杀药物系统进行联合治疗，拟建立一种具有特异性和高效性的恶性肿瘤靶向治疗方式。［方法］ 建立荷神经胶质瘤U87裸鼠模型30只，瘤体直径约14mm时，随机分为6组，每组5只：①生理盐水组；②131I-c(RGD)2 11.1MBq组；③131I-c(RGD)2 18.5MBq；④131I-c(RGD)2 18.5MBq+LV 组：连续2d，瘤体内注射方式将5×106U/25μl的重组慢病组载体E8-codA-GFP LV转染至肿瘤，然后通过尾静脉将18.5MBq 131I-c(RGD)2注入到小鼠体内；⑤131I-c(RGD)2 18.5MBq+LV+5-FC组：同④，将同量慢病毒载体转染至瘤内，同时腹腔注射10 mg前体药物5-FC连续7d。⑥131I-对照肽18.5MBq组（序列：YKAREC）。观察各组裸瘤肿瘤体积和质量的变化。［结果］ 治疗结束后，②、③、④、⑤、⑥组抑瘤率分别为36.75%、41.39%、43.63%、44.87%、3.31%。对瘤体质量进行比较，只有生理盐水组与③、④及⑤组之间差异有统计学意义（P＜0.05），而③、④及⑤组之间差异无统计学意义（P＞0.05）。［结论］ 131I-c(RGD)2能抑制U87神经胶质瘤的生长，对神经胶质瘤的治疗有潜在价值。但131I-c(RGD)2联合放射敏感性启动子E8和CD/5-FC自杀系统对于肿瘤细胞的治疗并没有达到统计学上的预期的效果，可能与开始治疗时的瘤体积过大或者样本量小有关。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of 131I-c(RGD)2 combined with lentiviral vector (LV) E8-codA-GFP in treatment of human glioblastoma in nude mice. ［Methods］ Thirty nude mice xenografted with human blioblastoma U87 MG were randomly divided into six groups. Group1 was injected with normal saline as control group；group 2 was injected with 131I-c(RGD)2 11.1 MBq； group3 was injected with 131I-c(RGD)2 18.5 MBq；in group 4 the xenografts were infected with the lentiviral vector by intratumor injection of 2×108 TU/25μL of E8-codA-GFP LV for 2 days and then were injected intravenously with 18.5 MBq 131I-c(RGD)2；in group 5 the 131I-c(RGD)2 18.5 MBq and LV were given as group 4，then 10mg prodrug 5-FC was injected i.p. for 7 days；group 6 was given 131I-scrambled peptide18.5 MBq. After 28 days the animals were sacrificed and the tumor volume，weight and net weight were measured in 6 groups. ［Results］ Compared to group 1，after 28 d of treatment the tumor inhibition rates in group 2，3，4，5 and 6 were 36.75%，41.39%，43.63%，44.87%，3.31%，respectively. There were significant differences in tumor weight between group 1 and groups 2，3，4，5 (P<0.05)，but no significant differences were observed among groups 2，3，4，5(P>0.05). ［Conclusion］ 131I-c(RGD)2 can effectively inhibit tumor growth in human glioblastoma tumor bearing mice，but no synergistic effect is observed when it is combined with radiation sensitive E8 promoter and CD/5-FC suicide gene system. |
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