| 曾 玮,陈 平.利用基因芯片重注释筛选肝细胞肝癌术后复发相关lncRNA[J].肿瘤学杂志,2017,23(8):658-662. |
| 利用基因芯片重注释筛选肝细胞肝癌术后复发相关lncRNA |
| Identification of lncRNAs Related to Postoperative Recurrence of Hepatocellular Carcinoma by Gene Microarray Re-annotation Strategy |
| 投稿时间：2016-12-06 |
| DOI：10.11735/j.issn.1671-170X.2017.08.B002 |
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| 中文关键词: 肝细胞肝癌 lncRNA 术后复发 预后 |
| 英文关键词:hepatocellular carcinoma(HCC) lncRNA postoperative recurrence prognosis |
| 基金项目:国家自然科学基金面上项目（30972895） |
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| 摘要点击次数: 2138 |
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| 中文摘要: |
| 摘 要：［目的］ 利用基因芯片重注释寻找肝细胞肝癌(hepatocellular carcinoma，HCC)术后复发相关lncRNA(long non-coding RNA)。［方法］ 首先利用生物信息的方式重新注释35/41例复发/未复发HCC病例的基因表达谱数据，筛查与HCC术后复发相关的差异表达lncRNA。然后利用ROC曲线评价差异lncRNA的术后复发预测效能。最后利用基因共表达网络与功能富集分析探究差异lncRNA具体的生物学功能。［结果］ 差异表达分析发现3个lncRNA(LINC00342，LINC00607，LINC01553)显著差异表达［|log(fold change)|>1，q value<0.05］。ROC曲线分析发现该3个差异lncRNA的AUC值为0.693。基因共表达网络分析发现LINC00342相关共表达网络显著富集到“细胞周期”相关功能；LINC00607相关的共表达网络中基因主要富集到“炎症反应”与“免疫反应”相关的生物学功能与通路；LINC01553相关的共表达网络中基因主要富集到“DNA复制与修复”相关的生物学功能与通路。［结论］ 3个lncRNA与HCC术后复发显著相关，可作为一种潜在的诊断标志物组合，值得进一步的挖掘与研究。 |
| 英文摘要: |
| Abstract：［Objective］ To identify the lncRNAs related to postoperative recurrence of hepatocellular carcinoma(HCC) by microarray re-annotation strategy. ［Methods］ The differentially expressed lncRNAs were identified in 35 cases of HCC with postoperative recurrence and 41 cases of HCC without recurrence by lncRNA re-annotation strategy. The classification power of the lncRNAs was analyzed by ROC curve. The biological functions and pathway related to these lncRNAs were investigated by gene co-expression network and gene functional enrichment analysis. ［Results］ Three differentially expressed lncRNAs(LINC00342，LINC00607，LINC01553) were found in the dataset ［(log(fold change) >1，q value<0.05)］. The AUC score of the three lncRNAs was 0.693 by ROC curve analysis. Co-expression network analysis and gene functional enrichment analysis showed that the genes in LINC00342 related co-expression network were enriched in cell cycle related biological functions；genes in LINC00607 related co-expression network were enriched in inflammatory response and immune response related biological functions；genes in LINC01553 related co-expression network were enriched in DNA replication and repair related biological functions. ［Conclusion］ The lncRNAs identified in the study may be involved in postoperative recurrence of HCC，which might be a potential biomarker for HCC prognosis and worth of further investigation. |
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