| 黄光明,沈 薇.SCD1对肝癌SMMC-7721细胞脂质代谢的影响及可能机制[J].肿瘤学杂志,2017,23(8):653-657. |
| SCD1对肝癌SMMC-7721细胞脂质代谢的影响及可能机制 |
| Effects of SCD1 on Lipid Metabolism in SMMC-7721 Cells and its Underlying Molecular Mechanism |
| 投稿时间：2017-01-23 |
| DOI：10.11735/j.issn.1671-170X.2017.08.B001 |
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| 中文关键词: SCD1 自噬 AMPK SMMC-7721细胞 脂质代谢 |
| 英文关键词:SCD1 autophagy AMPK SMMC-7721 cell lipid metabolism |
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| 中文摘要: |
| 摘 要：［目的］ 探讨SCD1对SMMC-7721细胞脂质代谢的影响及可能机制。［方法］ 肝癌细胞系SMMC-7721细胞培养，分为4组(对照组、CAY-10566处理组、CAY-10566联合Compound C共同处理组和CAY-10566联合氯喹共同处理组)，通过MTT法和流式细胞术分别检测细胞增殖和凋亡情况，透射电子显微镜观察细胞自噬体情况。胆固醇(CE)和甘油三酯(TAG)检测试剂盒分别检测上述4组SMMC-7721细胞的CE和TAG水平。［结果］抑制SCD1后SMMC-7721细胞自噬体明显增加，相应地减少了细胞活力，增加了细胞凋亡率；抑制SCD1基础上抑制AMPK或自噬后上述细胞的自噬体均明显减少，相应地增加了细胞活力，减少了细胞凋亡率。CE和TAG检测结果表明：抑制SCD1明显减少SMMC-7721细胞CE和TAG水平，与对照组相比，差异均有统计学意义(P<0.05)；抑制SCD1基础上抑制AMPK或自噬后SMMC-7721细胞CE和TAG水平均明显增加，与SCD1抑制剂组比较，差异均有统计学意义(P<0.05)。［结论］ SCD1可通过下调AMPK途径负性调节自噬，增加SMMC-7721细胞CE和TAG水平，抑制细胞凋亡，促进细胞生存，可能影响肝癌细胞的形成及进展。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate effects of SCD1 on lipid metabolism in SMMC-7721 cells and its underlying molecular mechanism. ［Methods］ SMMC-7721 cells were cultured and divided into four groups(the control group ，the CAY-10566 group，the group of CAY-10566 in combination with Compound C and the group of CAY-10566 concurrently with chloroquine)，and the cell viability and cell apoptosis were determined by MTT assay and flow cytometry，respectively，and the cell autophagosomes in the above cells were observed under a transmission electron microscope. The intracellular levels of cholesterol(CE) and triglycerides(TAG) in SMMC-7721 cells in the above four groups were assessed using CE and TAG assay kit. ［Results］ Inhibition of SCD1 significantly increased autophagosomes of the cytoplasm in SMMC-7721 cells，and accordingly decreased the cell viability and increased cell apoptosis. SCD1 activity was inhibited in combination with inhibition of AMPK signaling，or concurrently with autophagy inhibition，which led to significant reduction of the autophagosomes of the above cells，and accordingly increased the cell viability and decreased cell apoptosis. The data showed that inhibition of SCD1 led to a marked decrease in the levels of CE and TAG in SMMC-7721 cells(P<0.05)，and that inhibition of SCD1 in combination with inhibition of AMPK signaling，or concurrently with autophagy inhibition，caused a marked increase in their levels in the above cells(P<0.05). ［Conclusion］ SCD1 negatively regulated autophagy through inactivation of the AMPK signaling pathway to increase the SMMC-7721 cell levels of CE and TAG，and to suppress cell apoptosis and promote cell growth for the development and progression of hepatoma carcinoma cells. |
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