| 张艳辉,王炳高,邢志博.基于Hedgehog通路探究防己诺林碱对三阴性乳腺癌抗肿瘤机制的实验研究[J].肿瘤学杂志,2017,23(5):401-404. |
| 基于Hedgehog通路探究防己诺林碱对三阴性乳腺癌抗肿瘤机制的实验研究 |
| Anti-tumor Mechanism of Fangchinoline in Triple Negative Breast Cancer Based on Hedgehog Signaling Pathway |
| 投稿时间：2016-09-20 |
| DOI：10.11735/j.issn.1671-170X.2017.05.B008 |
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| 中文关键词: 防己诺林碱 三阴性乳腺癌 凋亡 Hedgehog信号通路 肿瘤 |
| 英文关键词:Fangchinoline triple negative breast cancer cell apoptosis hedgehog signaling pathways tumor |
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| 摘要点击次数: 2103 |
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| 中文摘要: |
| 摘 要：［目的］ 探究防己诺林碱对三阴性乳腺癌抗肿瘤机制。［方法］ MTT法检测防己诺林碱对三阴性乳腺癌细胞的抑制率，计算IC50；细胞流式实验观察防己诺林碱对三阴性乳腺癌细胞的凋亡影响；Western blot法探究防己诺林碱对Smo及Gli1蛋白表达的影响。［结果］ 防己诺林碱对三阴性乳腺癌细胞BT549的活力有影响，IC50值为8.99μmol/L。随着防己诺林碱浓度升高细胞凋亡率增加，三阴性乳腺癌细胞BT549通路Hedgehog中的Smo、Gli1蛋白表达水平明显降低（P<0.05）。FAN可以抑制肿瘤的生长。［结论］ 防己诺林碱可通过下调细胞凋亡相关信号通路Hedgehog来影响三阴性乳腺癌细胞凋亡，同时影响三阴性乳腺癌肿瘤的生长。 |
| 英文摘要: |
| Abstract：［Objective］ To explore the anti-tumor mechanism of Fangchinoline in triple negative breast cancer based on hedgehog signaling pathway. ［Methods］ Three negative breast cancer (TNBC) BT549 cells were treated with fangchinoline. The inhibition rate of BT549 cells was detected by MTT and IC50 was calculated; the cell apoptosis was detected by flow cytometry. The expression of Smo and Gli1 protein was detected by Western blot. ［Results］ Fangchinoline inhibited the proliferation of BT549 cells with a IC50 of 8.99μmol/L. Flow cytometry showed that Fangchinoline induced the apoptosis of BT549 cells in a dose-dependent manner. The expression of Smo and Gli1 in the BT549 cells was declined with the increasing Fangchinoline concentration. Fangchinoline also inhabited the growth of BT549 breast cancer grafts in tumor-bearing nude mice. ［Conclusion］ Fangchinoline inhibits cell growth and enhances cell apoptosis of TNBC cells in vitro and in vivo，which may be associated with the regulation by hedgehog signaling pathway. |
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