| 林伟仁,庞梦霞,朱 锋.Akt抑制剂哌立福新通过减少乳酸生成抑制胃癌细胞增殖与迁移[J].肿瘤学杂志,2017,23(5):394-400. |
| Akt抑制剂哌立福新通过减少乳酸生成抑制胃癌细胞增殖与迁移 |
| Perifosine，an Akt Inhibitor，Suppress the Proliferation and Migration via Decreasing Lactate Production in Gastric Cancer Cells |
| 投稿时间：2017-01-29 |
| DOI：10.11735/j.issn.1671-170X.2017.05.B007 |
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| 中文关键词: Akt抑制剂 哌立福新 胃癌细胞 增殖 迁移 糖酵解 |
| 英文关键词:Akt inhibitor perifosine gastric cancer cells proliferation migration glycolysis |
| 基金项目:国家自然科学基金（81371429），杭州市科技局项目（20140633B37） |
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| 中文摘要: |
| 摘 要：［目的］ 探究Akt抑制剂哌立福新对胃癌细胞增殖、凋亡及迁移的作用及其可能机制。［方法］ 采用不同浓度哌立福新处理胃癌MGC803和SGC7901细胞。细胞增殖采用磺酰罗丹明B法检测，流式细胞仪Annexin V-/PI双染法检测细胞凋亡，细胞迁移和侵袭分别采用细胞划痕实验法和Transwell小室实验检测，乳酸含量的测定采用ELISA法，以蛋白印迹法检测糖酵解通路相关蛋白水平的变化。［结果］ 哌立福新在2.0 μmol/L浓度剂量时即能有效抑制胃癌细胞MGC803和SGC7901细胞增殖。经哌立福新低中高剂量（2.0、10.0、20.0 μmol/L）处理后，MGC803细胞凋亡明显增加（P<0.05）。划痕间距分别为407.2±34.4 μm，657.2± 49.2μm和910.8±51.4.1μm，与对照组240.3±27.8 μm相比均有显著性差异（P<0.05），且呈量效关系。Transwell小室实验显示每个视野下侵袭的MGC803细胞数对照组为2584±228个，而经哌立福新低中高剂量处理后分别为2052±158 (P<0.05)，1410±105(P<0.01) 和887±87 (P<0.01)，与对照组相比均有显著性差异，并呈剂量依赖性。不同剂量哌立福新均可显著性降低培养基和细胞中乳酸含量，同时，蛋白质印迹结果提示哌立福新显著性抑制与糖酵解乳酸生成相关的乳酸脱氢酶-A，葡萄糖转运蛋白（Glucose transporter，GLUT）1，GLUT4和IGF-1的表达（P<0.05），且呈剂量依赖性。［结论］ 哌立福新能有效抑制MGC803细胞迁移与侵袭，其机制与降低糖酵解从而减少乳酸生成相关。 |
| 英文摘要: |
| Abstract：［Objective］ To explore the effect and its potential mechanism of perifosine，an Akt inhibitor，on cell proliferation，apoptosis and migration in gastric cancer cells. ［Methods］ MGC803 and SGC7901 cells were treated with perifosine at different doses. Cell proliferation was detected by sulforhodamine B assay. Cell apoptosis was assayed by Annexin V-/PI kit using flow cytometry. Wound healing and transwell chamber assays were conducted to detect cell migration and invasion，respectively. The concentration of lactic acid in culture medium and cell lysate was detected by ELISA，and protein expression was detected by western blot analysis. ［Results］ Perifosine at the dose of 2 μmol/L had inhibitive effect on cell proliferation in gastric cell line MGC803 and SGC7901. MGC803 cells treated with perifosine at the dose of 2，10，and 20 μmol/L displayed obvious apoptotic rate（P<0.05）. Wound healing assay revealed that the scratch space in perifosine-treated groups was significantly increased in a dose-dependent manner in MGC803 cells(407.2±34.4μm，P<0.05，657.2±49.2 μm，P<0.01，and 910.8±51.4.1μm，P<0.01，in low，medium and high dose，respectively) as compared to control group(240.3±27.8μm). Transwell assay exhibited that migrated cells in control group was 2584±228，while those in perifosine-treated groups were 2052±158(low，P<0.05)，1410±105 (medium，P<0.01) and 887±87(high，P<0.01). In addition，perifosine dose-dependently suppressed lactate production detected both in culture medium and in cell lysate. Consistently，the protein level of lactate dehydrogenase A(LDH-A) and other important proteins which regulate glycolysis such as glucose transporter(GLUT) 1，GLUT4 and IGF were also dose-dependently inhibited by perifosine treatment. ［Conclusion］ Perifosine significantly inhibits cell migration and invasion in gastric cancer MGC803 cells，which may be resulted from its regulative effect on glycolysis and subsequent decrease of lactic acid. As perifosine has been studied in clinical Phase Ⅱ or Ⅲ trials in various tumors，it is a potential drug in treating gastric cancer. |
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