| 郑传铭,陆晓筱,凌志强.甲状腺乳头状癌组织中TSHR基因启动子甲基化及其临床意义[J].肿瘤学杂志,2017,23(4):257-261. |
| 甲状腺乳头状癌组织中TSHR基因启动子甲基化及其临床意义 |
| Methylation of TSHR Gene Promoter in Papillary Thyroid Carcinoma and its Clinical Significance |
| 投稿时间：2016-11-28 |
| DOI：10.11735/j.issn.1671-170X.2017.04.B001 |
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| 中文关键词: 甲状腺乳头状癌 TSHR基因 甲基化 甲基化特异性实时聚合酶链反应 |
| 英文关键词:thyroid papillary carcinoma TSHR gene methylation methylation-specific real-time polymerase chain reaction |
| 基金项目:浙江省自然科学基金项目(LY17H280003)；浙江省医药卫生科技计划项目（2010KYA031；2016KYA054） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨甲状腺乳头状癌（papillary thyroid cancer，PTC）组织中TSHR基因启动子区甲基化程度及其临床意义。［方法］采用甲基化特异性聚合酶链反应（Methylation-Specific Polymerase chain reaction，MSP）法检测100例PTC组织及其癌旁组织中TSHR基因启动子甲基化状态，并结合患者的临床病理特征和随访信息分析TSHR与PTC进展及预后的关系。［结果］ 100例PTC组织中TSHR基因启动子甲基化率为77%，明显高于癌旁组织的7%（P=0.002）。TSHR基因甲基化程度与患者初诊年龄、原发灶大小、数量、腺外侵犯、淋巴转移、TNM分期呈正相关（P<0.05）；而与患者性别、T分期无关（P>0.05）。单因素生存分析显示TSHR基因甲基化预示更短的无病生存时间，而多因素生存分析显示TSHR基因甲基化不是PTC患者无病生存的独立影响因素。［结论］ TSHR基因启动子区甲基化是PTC组织中频发的分子事件，与肿瘤的进展相关并提示PTC患者的不良预后。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the methylation status of TSHR gene promoter region in thyroid papillary thyroid carcinoma(PTC) and its clinical significance.［Methods］ The methylation status of TSHR gene promoter was detected by methylation-specific polymerase chain reaction (MSP) in 100 cases of PTC tissue and the paired adjacent normal tissues. The correlation of TSHR methylation with the tumor progression and prognosis was analyzed. ［Results］ The methylation rate of TSHR gene promoter in PTC was 77%，which was significantly higher than that in matched normal tissue(P=0.002). There was a positive correlation of the methylation of TSHR gene with the age，the size of primary tumor，the number of primary tumor，the tumor invasion，lymphatic metastasis and TNM stage(P<0.05)，but not with gender and T stage (P>0.05). Univariate survival analysis showed that TSHR gene methylation was associated with shorter disease-free survival time. Multivariate survival analysis showed that TSHR gene methylation was not associated with the survival of patients. ［Conclusion］ The methylation of TSHR gene promoter region is a frequent molecular event in PTC tissue，which is associated with the tumor progression and may suggest a poor prognosis in PTC patients. |
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