| 王成双,佐满珍.华蟾素联合顺铂对卵巢癌细胞A2780增殖及凋亡的影响[J].肿瘤学杂志,2017,23(1):30-34. |
| 华蟾素联合顺铂对卵巢癌细胞A2780增殖及凋亡的影响 |
| Effect of Cinobufacini and Cisplatin on Cell Proliferation and Apoptosis in Human Ovarian Carcinoma Cell Line A2780 |
| 投稿时间：2016-02-11 |
| DOI：10.11735/j.issn.1671-170X.2017.01.B006 |
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| 中文关键词: 华蟾素 顺铂 细胞增殖 细胞凋亡 卵巢肿瘤 A2780细胞 |
| 英文关键词:Cinobufacini cisplatin cell proliferation apoptosis ovarian neoplasms A2780 |
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| 中文摘要: |
| 摘 要：［目的］ 研究华蟾素联合顺铂对卵巢癌细胞A2780增殖、凋亡的影响。［方法］ 采用四甲基偶氮唑盐微量酶反应比色法（MTT法）检测华蟾素联合顺铂对卵巢癌细胞A2780的半数抑制浓度（IC50）和细胞生长抑制率，采用流式细胞术检测细胞周期，Hoechst染色检测细胞凋亡。［结果］华蟾素及顺铂能抑制细胞增殖，华蟾素IC50为0.56mg/ml；顺铂IC50为2.8μg/ml；药物对癌细胞的生长抑制率随时间延长而增强。华蟾素和顺铂对A2780细胞表现出凋亡作用，华蟾素使A2780细胞周期停滞于S期(P＜0.01)，顺铂使A2780细胞周期停滞于G0/G1期（P＜0.05）。［结论］ 华蟾素联合顺铂可诱导A2780细胞周期阻滞和细胞凋亡，从而抑制A2780细胞生长。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of Cinobufacini and cisplatin on cell proliferation and apoptosis in ovarian cancer A2780 cells. ［Methods］ Ovarian cancer A2780 cells were treated with Cinobufacini and cisplatin in vitro. IC50 and inhibition rate of A2780 cells were detected with MTT assay，and the cell cycles and cell apoptosis were detected with flow cytometry and Hoechst staining，respectively. ［Results］ Cinobufacinin and cisplatin showed inhibitory effect on A2780 cells in a time-dependent manner，the IC50 of Cinobufacini and cisplatin were 0.56mg/ml and 2.80μg/ml respectively. Cinobufacini and cisplatin induced apoptosis of ovarian cancer A2780 cells. Cinobufacini arrested A2780 cell cycles in S phase(P＜0.01)，while cisplatin arrested A2780 cell cycles in G0/G1 phase(Ｐ＜0.05). ［Conclusion］ Cinobufacini and cisplatin can inhibit the proliferation of ovarian cancer A2780 cells by inducing cell cycle arrest and cell apoptosis. |
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