| 林白桦,贾勇士,毕爱红.吉非替尼在小鼠肺照射后不同时段给药对肺损伤影响[J].肿瘤学杂志,2017,23(1):13-19. |
| 吉非替尼在小鼠肺照射后不同时段给药对肺损伤影响 |
| Effect of Gefitinibon Radiation-Induced Lung Injury in C57 Mice after Different Time Administration |
| 投稿时间：2016-09-18 |
| DOI：10.11735/j.issn.1671-170X.2017.01.B003 |
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| 中文关键词: 吉非替尼 肺损伤 羟脯氨酸 转化生长因子-β1 |
| 英文关键词:gefitinib lung injury hydroxyproline transforming growth factor-β1 |
| 基金项目:浙江省医药卫生一般研究计划（2013KYB027） |
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| 中文摘要: |
| 摘 要：［目的］ 观察不同时段给予吉非替尼对C57BL 小鼠放射性肺损伤的影响。［方法］ 将128只C57BL雌性小鼠随机分组分为8组：（1）照射+早期予生理盐水组（A组）16只，全肺单次照射13Gy，在照射后立即第一次给生理盐水，以后每天均灌胃给药至第15d；(2)照射+晚期予生理盐水组（B组）16只，全肺单次照射13Gy，在照射后第15d第一次给生理盐水，以后每天均灌胃给药至第30d；（3）早期给予生理盐水组（C组）16只，在照射组照射后立即第一次给生理盐水，以后每天均灌胃给药至第15d；（4）晚期给予生理盐水组（D组）16只，在照射组照射后第15d第一次给生理盐水，以后每天均灌胃给药至第30d；（5）早期给药组（E组）16只，在照射组照射后立即第一次给药，以后每天均灌胃给药至第15d，给药剂量200mg/kg·d；（6）晚期给药组（F组）16只，在照射组照射后第15d第一次给药，以后每天均灌胃给药至第30d，给药剂量200mg/kg·d；（7）照射+早期给药组（G组）16只，全肺单次照射13Gy后立即第一次给药，以后每天均灌胃给药至第15d，给药剂量200mg/kg·d；（8）照射+晚期给药组（H组）16只，全肺单次照射13Gy后第15d第一次给药，以后每天均灌胃给药至第30d，给药剂量200mg/kg·d。在照射后30d、60d处死小鼠后取部分肺组织HE染色，检测血清转化生长因子β1（transforming growth factor-β1，TGF-β1）水平、以及部分肺组织羟脯氨酸（hydroxyproline，HYP）水平。［结果］ 病理学检查显示，照射+晚期给药组（H组）在照射后60d时较其他照射组肺部损伤反应较轻。统计分析提示，各组之间TGF-β1和HYP水平比较有统计学差异（P均＜0.01）。照射、给药、时间因素对小鼠血清中TGF-β1和HYP水平有统计学差异（P＜0.05）。［结论］200mg/kg·d给药剂量情况下吉非替尼未显示明显的与照射累加的肺损伤，照射后晚期给予吉非替尼具有减轻放射性肺损伤作用。 |
| 英文摘要: |
| Abstract：［Objective］ To explore the effect of gefitinib on radiation-induced lung injury by administered it to C57 mice which had whole lung irradiation at different time periods postirradiation. ［Methods］ One hundred twenty-eight mice C57/BL(female) were divided into 8 groups by random digits table method. There were 16 mice in every groups. Irradiation + normal saline group at early phase(A)，irradiation + normal saline group at late phase(B)，irradiation + gefitinib group at early phase (G) and irradiation +gefitinib group at late phase(H) were irradiated first. Irradiation method：10MV-X ray，via the anterior chest，whole lung irradiation with 13Gy by single dose. Drug delivering method：Group A and G were given saline or gefitinib(200mg/kg·d) by gavage after irradiation on day 0 to day 15. Group B and H were given saline or gefitinib(200mg/kg·d) after irradiation on day 15 to day 30，once a day. Normal saline group at early phase(group C)，normal saline group at late phase(group D)，gefitinib group at early phase(group E) and gefitinib at late phase(group F) were given saline or gefitinib(200mg/kg·d) by gavage after other groups were irradiated at corresponding time periods. ［Results］ The inflammation in group H was lighter than that in the other groups which had been irradiated at 60 days. Gefitinib，irradiation and time factor had significant effect about TGF-β1 levels of serum(P＜0.05). Time and irradiation factor had significant effect about HYP levels of lung tissue(P<0.01).［Conclusions］ It doesn’t shown additive lung injury when given gefitinib(200mg/kg·d) after irradiation. Gefitinib could lighten radiation-induced lung injury when it is given at right time after irradiated. |
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