| 朱光辉,周联明,王时光.HIF-1α调控Snail基因启动子活性及作用位点的鉴定[J].肿瘤学杂志,2016,22(11):911-916. |
| HIF-1α调控Snail基因启动子活性及作用位点的鉴定 |
| Modulating Effects of HIF-1α on the Promoter Activity of Snail and the Interaction Site Evaluation |
| 投稿时间：2016-03-02 |
| DOI：10.11735/j.issn.1671-170X.2016.11.B009 |
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| 中文关键词: 缺氧诱导因子-1α Snail 电泳迁移率实验 染色质免疫沉淀 |
| 英文关键词:hypoxia inducible factor-1α Snail EMSA ChIP |
| 基金项目:上海市奉贤区科学技术委员会科学技术发展（奉科20131304） |
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| 中文摘要: |
| 摘 要： ［目的］ 探讨缺氧诱导因子-1α（HIF-1α）对Snail启动子活性的作用，并鉴定其作用位点。［方法］ 应用transfac软件分析Snail启动子区域潜在的HIF-1α结合位点。通过荧光素酶报告基因载体、电泳迁移率实验（EMSA）和染色质免疫沉淀（ChIP）实验研究HIF-1α是否能够与Snail启动子区域潜在的位点结合，直接调控Snail的表达。［结果］ 生物信息学分析发现Snail启动子区域存在2个HIF-1α可能的结合位点（-653，-649）和（-543，-538）。双荧光素酶报告基因检测发现（-543，-538）位点是HIF-1α特异性结合于Snail的位点，HIF-1α蛋白与之结合后调节Snail基因转录活性。EMSA和ChIP实验证实了HIF-1α蛋白和Snail启动子区（-543，-538）结合，直接上调Snail的表达。［结论］ Snail启动子区域存在HIF-1α的结合位点，HIF-1α与之结合后直接调控Snail的表达。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effects of HIF-1α on the promoter activity of Snail and to identify the interaction site. ［Methods］ Transfac software was used to analyze the potential HIF-1α binding sites in region of Snail promoter. Luciferase reporter gene system，EMSA and ChIP were used to identify the interaction of HIF-1α and Snail.［Results］ Snail promoter region was found to be 2 potential interaction sites：(-653，-649) and (-543，-538). Dual luciferase reporter gene assay clarify(-543，-538) was the site of HIF-1α specific binding to Snail. EMSA and ChIP experiments confirmed that Snail promoter region (-543，-538) was the interaction site by HIF-1α. ［Conclusion］ Snail promoter region has a interaction site by HIF-1α，and the expression of Snail is regulated by HIF-1α. |
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