| 李 沛,陈 丹,赵正维.miR-320a抑制结肠癌细胞增殖及肺转移作用的研究[J].肿瘤学杂志,2016,22(10):794-800. |
| miR-320a抑制结肠癌细胞增殖及肺转移作用的研究 |
| The Study of miR-320a in Colon Cancer and Its Inhibitory Effects on Proliferation and Pulmonary Metastasis |
| 投稿时间：2016-04-18 |
| DOI：10.11735/j.issn.1671-170X.2016.10.B004 |
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| 中文关键词: 结肠肿瘤 肺转移 miR-320a 增殖 侵袭 |
| 英文关键词:colon neoplasms pulmonary metastasis microRNA-320a proliferation invasion |
| 基金项目:唐都医院科技创新发展基金（2015JCYJ001） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨调节结肠癌细胞中miR-320a的表达水平后，其对结肠癌细胞生物学行为的影响及可能作用机制。［方法］ 检测调节miR-320a结肠癌细胞的表达水平后，用MTT法检测细胞增殖能力的变化，Transwell侵袭实验检测细胞侵袭力变化，通过给裸鼠尾静脉注射miR-320a不同表达水平的结肠癌细胞后，观察裸鼠肺转移的情况；通过生物信息学软件预测miR-320a的潜在靶基因并通过双荧光素酶报告基因系统进行鉴定，Western Blot法检测β-catenin、MMP-2、MMP-9、E-cadherin、N-cadherin和Vimentin的蛋白表达情况。［结果］ 通过慢病毒感染miR-320a质粒上调结肠癌细胞中miR-320a水平后，可以抑制结肠癌细胞增殖、抑制结肠癌细胞侵袭及肺转移，β-catenin是miR-320a的靶基因，miR-320a下调β-catenin、MMP-2、MMP-9、N-cadherin和Vimentin表达，上调E-cadherin表达。［结论］ miR-320a可以抑制结肠癌细胞的增殖及侵袭转移。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the role of miR-320a in colon cancer and its effect on the biological behavior of colon cancer cells. ［Methods］ Colon cancer was infected cell with constructed highly-express miR-320a plasmid. Proliferation，invasion and pulmonary metastasis of infected cells were measured by MTT assay，transwell assay and nude mouse，respectively. Computational analysis tool and luciferase reporter assay were used to predict and verify target of miR-320a. The expression of β-catenin，MMP-2，MMP-9，E-cadherin，N-cadherin and Vimentin were detected by Western Blot. ［Results］ MiR-320a inhibited proliferation，invasion and pulmonary metastasis in colon cancer. Expression of β-catenin，MMP-2，MMP-9，N-cadherin and Vimentin were down-regulated after up-regulated miR-320a，except E-cadherin. ［Conclusion］ The miR-320a inhibits proliferation，invasion and pulmonary metastasis in colon cancer. |
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