| 陈 飞,冯玉光,任盼盼.Hp感染与胃癌组织中HIF-2α、Oct-4表达的相关性[J].肿瘤学杂志,2016,22(10):789-793. |
| Hp感染与胃癌组织中HIF-2α、Oct-4表达的相关性 |
| The Correlation of Helicobacter Pylori Infection and Expression of HIF-2α，Oct-4 in Gastric Carcinoma Tissues |
| 投稿时间：2016-01-12 |
| DOI：10.11735/j.issn.1671-170X.2016.10.B003 |
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| 中文关键词: 胃肿瘤 Hp HIF-2α Oct-4 |
| 英文关键词:gastric neoplasms helicobacter pylori hypoxia inducible factor-2α Octamer-4 |
| 基金项目: |
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| 摘要点击次数: 1762 |
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| 中文摘要: |
| 摘 要：［目的］ 通过研究胃癌组织中Hp感染与HIF-2α、Oct-4蛋白表达的相关性，初步探讨Hp感染在胃癌发生发展中的干细胞机制及对化疗抵抗的分子机制。［方法］ 采用快速尿素酶试验和免疫组织化学SP法分别检测80例行胃癌手术切除的组织标本（研究组）和60例慢性胃炎患者经胃镜下取组织活检标本（对照组）中的Hp，采用免疫组织化学SP法检测Hp阳性和Hp阴性的研究组组织中HIF-2α、Oct-4蛋白的表达。［结果］ 研究组Hp阳性率明显高于对照组（χ2=5.865，P=0.012）。研究组中T3+T4 Hp阳性率明显高于T1+T2（χ2=9.113，P=0.003），有淋巴结转移Hp阳性率明显高于无淋巴结转移（χ2=6.236，P=0.013）。研究组中Hp阳性的HIF-2α蛋白阳性表达率明显高于Hp阴性，Hp感染与HIF-2α蛋白表达呈正相关（r=0.311，P=0.007）；Hp阳性的Oct-4阳性表达率明显高于Hp阴性，Hp感染与Oct-4蛋白表达呈正相关（r=0.324，P=0.005），HIF-2α蛋白表达与Oct-4蛋白表达呈正相关（r=0.560，P=0.000）。［结论］ Hp可能通过激活HIF-2α-Oct-4通路来促进胃癌组织中HIF-2α和Oct-4的表达，进而诱导胃癌细胞的干细胞化及化疗抵抗。 |
| 英文摘要: |
| Abstract：［Objective］To observe the correlation of Hp infection and the expression of HIF-2α and Oct-4 in gastric carcinoma tissues，in order to discuss whether Hp infection effect in the initiation and development of gastric cancer and inducing chemotherapy resistance.［Methods］ Hp infection was identified by rapid urease test and immunocytochemistry in gastric cancer resection was detected in 80 tissue samples (research group) and 60 patients with chronic gastritis by gastroscope take biopsy specimen under normal (control group)．Immunohistochemistry was used to detect the expression of HIF-2α and Oct-4 proteins in gastric carcinoma (tissues were divided into Hp positive group and Hp negative group).［Results］ Hp positive infection rate in research group was significantly higher than that in control group(χ2=5.856，P=0.012).Hp positive rate of patients with T3，T4 in research group was significantly higher than that of patients with T1 and T2（χ2=9.113，P=0.003).Lymphatic metastasis was significantly higher than without lymph node metastasis in Hp positive rate（χ2=6.236，P=0.013). The expression rate of HIF-2 in Hp positive group was significantly higher than that in the negative in research group.Expression of Hp infection and HIF-2 alpha protein was positively correlated（r=0.311，P=0.007).The expression of Oct-4 in Hp positive rate was significantly higher than that in negative.Expression of Hp infection and Oct-4 protein was positively correlated(r=0.324，P=0.005).HIF-2 alpha protein expression and Oct-4 protein expression were positively correlated(r=0.560，P=0.000).［Conclusion］ Hp can stimulate the expression of HIF-2α and Oct-4 in gastric carcinoma tissues by activating HIF-2α-Oct-4 pathway，suggesting that Hp may play an important role in inducing gastric carcinoma cell de-differentiation and chemotherapy resistance. |
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