| 张志栋,李 勇,檀碧波.抑制膜联蛋白A2表达对人胃癌细胞株裸鼠移植瘤生长的影响及机制[J].肿瘤学杂志,2016,22(9):693-697. |
| 抑制膜联蛋白A2表达对人胃癌细胞株裸鼠移植瘤生长的影响及机制 |
| Effect of ANXA2 on Growth of Implanted Human Gastric Cancer in Nude Mice |
| 投稿时间：2016-04-05 |
| DOI：10.11735/j.issn.1671-170X.2016.09.B001 |
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| 中文关键词: 胃肿瘤 膜联蛋白A2 肿瘤生长 裸鼠 |
| 英文关键词:gastric neoplasms Annexin A2 tumor growth nude mice |
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| 中文摘要: |
| 摘 要：［目的］ 通过体内研究探讨膜联蛋白A2（ANXA2）对胃癌生长的影响及相关作用机制。［方法］ 常规培养人胃癌细胞株BGC-823；将细胞接种到裸鼠皮下制备移植瘤模型。制模成功后随机分组，分别以脂质体/ANXA2-siRNA复合物（实验组）、脂质体/Non-siRNA复合物（对照组）或生理盐水（空白组）分别在各组肿瘤局部注射，每4d干预1次。干预4次后处死，绘制肿瘤生长曲线并比较瘤重。应用荧光定量RT-PCR及免疫组化技术检测各组ANXA2、增殖细胞核抗原（PCNA）、p27mRNA和蛋白表达。［结果］ 裸鼠皮下移植瘤造模成功率100.00%（15/15）。实验组肿瘤生长比其他两组缓慢，最终体积实验组为（1680.26±110.51）mm3，对照组为（2194.18±188.48）mm3，空白组（2172.37±212.36）mm3（F=9.341，P=0.004）；肿瘤重量实验组（20.63±0.46）g，对照组为（22.92±0.86）g，空白组为（23.44±0.90）g（F=11.688，P=0.002）。PCR及免疫组化结果显示实验组ANXA2、PCNAmRNA和蛋白表达明显低于其他两组（P<0.05），而p27mRNA和蛋白表达明显高于其他两组（P<0.01）。［结论］ 抑制ANXA2基因表达后胃癌移植瘤的生长明显受到抑制，这可能与ANXA2基因调控PCNA、p27表达有关。 |
| 英文摘要: |
| Abstract： ［Objective］ To investigate the effect of Annexin A2(ANXA2) on the growth of gastric cancer in vivo. ［Methods］ Human gastric cancer BGC-823 cells were inoculated into 15 nude mice subcutaneously to establish gastric cancer model. The cancer-bearing nude mice were divided into 3 groups randomly with 5 in each group，the animals were treated with liposome/ANXA2-siRNA compound(ANXA2 group)，liposome/non-siRNA compound(control group) or normal saline(blank group)，respectively. After nude mice were sacrificed，volume and weight of tumors were measured，and the expressions of ANXA2，PCNA，p27 mRNA and protein were detected with fluorescence quantitative RT-PCR and immunohistochemistry，respectively. ［Results］ The tumor growth in ANXA2 group was slower than that in other 2 groups; and the final volume of tumors was（1680.26±110.51）mm3 in ANXA2 group，（2194.18±188.48）mm3 in control group and (2172.37±212.36）mm3 in blank group（F=9.341，P=0.004）. The weight of tumors was(20.63±0.46）g in ANXA2 group，（22.92±0.86）g in control group and(23.44±0.90）g in blank group（F=11.688，P=0.002）. The expressions of ANXA2 and PCNA mRNA and protein in ANXA2 group were lower than those in other 2 groups，whereas expressions of p27 mRNA and protein in ANXA2 group were higher than those in the other 2 groups（P<0.01）. ［Conclusion］ The growth of tumor can be inhibited after ANXA2 inhibition，indicating that ANXA2 may regulate expressions of PCNA and p27 in gastric cancer. |
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