| 李 优,王 剑,牟 好.姜黄素通过PI3K/AKT/mTOR通路抑制TGF-β1诱导的肺癌细胞上皮间质转化[J].肿瘤学杂志,2016,22(8):607-614. |
| 姜黄素通过PI3K/AKT/mTOR通路抑制TGF-β1诱导的肺癌细胞上皮间质转化 |
| Curcumin Inhibits TGF-β1-induced Epithelial-mesenchymal Transition of Lung Adenocarcinoma Cells via PI3K/AKT/mTOR Signaling Pathway |
| 投稿时间：2016-03-20 |
| DOI：10.11735/j.issn.1671-170X.2016.08.B001 |
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| 中文关键词: 姜黄素 肺肿瘤 上皮间质转化 TGF-β1 |
| 英文关键词:curcumin lung neoplasms epithelial-mesenchymal transition transforming growth factor β1 |
| 基金项目:南京军区医学科技创新项目(15MS158)；浙江省科技厅公益性技术应用研究计划(2014C33277)；杭州市科技发展计划项目(20130633B29，20140633B40) |
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| 中文摘要: |
| 摘 要：［目的］ 探讨姜黄素对TGF-β诱导的肺癌A549细胞上皮间质转化、侵袭转移的影响及其可能的机制。［方法］ 通过转化生长因子TGF-β1诱导肺癌细胞株A549发生上皮间质转化；利用不同浓度姜黄素干预由TGF-β1诱导的肺癌 A549细胞，倒置显微镜观察细胞形态变化，细胞划痕实验、Transwell 侵袭实验检测细胞迁移及侵袭能力变化，Western blot法检测上皮表型标记蛋白E-cadherin和间质表型标记蛋白N-cadherin、Vimentin的表达及PI3K/AKT/mTOR信号通路中AKT、mTOR磷酸化的情况，并利用PI3K抑制剂LY290004、mTOR抑制剂Rapamycin通过上述方法对姜黄素的作用进行印证。［结果］ 与对照组相比，姜黄素显著增加A549细胞E-cadherin的表达，抑制N-cadherin和Vimentin蛋白及TGF-β1刺激p-AKT和p-mTOR的表达，且呈明显的剂量—时间依赖关系；同时抑制TGF-β诱导的侵袭迁移。［结论］ 姜黄素可通过PI3K/AKT/mTOR通路明显抑制TGF-β1诱导的肺癌A549细胞上皮间质转化，降低其侵袭迁移能力。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of curcumin on TGF-β1-induced epithelial-mesenchymal transition，invasion and migration of lung cancer A549 cells，and the potential mechanism. ［Methods］ Different concentrations of curcumin were used to intervene the TGF-β1 treated lung cancer A549 cells. The morphological changes were observed under inverted microscope；wound Healing assay and Transwell assay were used to observe the changes if invasion and migration ability of A549 cells；Western blot was used to analyze the expression of epithelial phenotype marker protein E-cadherin，mesenchymal phenotype marker protein N-cadherin，Vimentin and the phosphorylation of transcription factor AKT，mTOR in PI3K/AKT/mTOR signaling pathway. PI3K inhibitors and mTOR inhibitors were applied to conform the affects of curcumin.［Results］ Compared with controls，curcumin inhibited the invasion and migration of TGF-β1-induced A549 cell；decreased the expression of N-cadherin，Vimentin；increased the expression of E-cadherin；suppressed the expression of p-AKT and p-mTOR stimulated by TGF-β1，in a dose and time-dependent manner.［Conclusion］ Curcumin can inhibit TGF-β1-induced epithelial-mesenchymal transition of lung adenocarcinoma cells，in which PI3K/AKT/mTOR signaling pathway may be involved. |
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