| 王丽君,肖仲清,姚 亮.JQ1下调SKP2和上调p27诱导HeLa细胞凋亡的研究[J].肿瘤学杂志,2016,22(7):533-537. |
| JQ1下调SKP2和上调p27诱导HeLa细胞凋亡的研究 |
| Study on JQ1 Inducing Apoptosis by Down-regulation of SKP2 and Up-regulation of p27 in HeLa Cells |
| 投稿时间：2015-09-19 |
| DOI：10.11735/j.issn.1671-170X.2016.07.B002 |
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| 中文关键词: JQ1 HeLa细胞 细胞凋亡 S期激酶相关蛋白2 p27 |
| 英文关键词:JQ1 HeLa cells cell apoptosis S-phase kinase-associated protein 2(SKP2) p27 |
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| 中文摘要: |
| 摘 要：［目的］ 观察JQ1对宫颈癌HeLa细胞增殖的影响及可能的作用机制。［方法］ 采用CCK-8比色法检测JQ1对HeLa细胞增殖的影响，分为二甲亚砜（DMSO）对照组和JQ1处理组（终浓度分别为0.01、0.1、1和10μmol/L），分别处理24、48、72和120h。将细胞分为DMSO对照组和10μmol/L JQ1处理组进行后续实验，细胞培养12d后计算细胞克隆形成数量；细胞培养72h后采用流式细胞仪检测细胞凋亡的变化，采用实时荧光定量PCR（RT-qPCR）和蛋白印迹法检测S期激酶相关蛋白2（SKP2）和p27的表达变化。［结果］ JQ1抑制HeLa细胞增殖，且作用呈剂量与时间依赖性，10μmol/L JQ1处理细胞72h后细胞存活数量减半；与DMSO对照组相比，10μmol/L JQ1显著抑制HeLa细胞克隆形成（细胞克隆形成数量：3±2 vs 240±10，P<0.001）；且能诱导HeLa细胞凋亡（细胞凋亡百分比：12.80±0.88 vs 2.90±0.27，P< 0.01）；与DMSO对照组相比，JQ1能抑制细胞SKP2 mRNA 和蛋白表达（其中SKP2 mRNA表达倍数：0.43±0.02 vs 1.00±0.03，P<0.01），并上调p27 mRNA 和蛋白表达（p27 mRNA表达倍数：2.60±0.13 vs 1.00±0.11，P<0.01）。［结论］ JQ1通过诱导细胞凋亡来抑制HeLa细胞的增殖，其可能的机制是抑制SKP2表达和上调p27表达。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effect of JQ1 on proliferatoin of HeLa cells and its potential mechanism．［Methods］ HeLa cells were exposed to dimethyl sulfoxide (DMSO，control group) or different concentrations of JQ1(0.01，0.1，1 and 10μmol/L，JQ1 group) in the culture medium for 24，48，72 and 120h respectively，and cell proliferation analysis was performed using CCK-8 method. HeLa cells were exposed to DMSO or 10μmol/L JQ1 in the culture medium for 12d，and the number of colony formation was assayed by counting. HeLa cells were exposed to DMSO or 10μmol/L JQ1 in the culture medium for 72h，and the apoptosis was assayed by flow cytometry，and the expression of S-phase kinase-associated protein 2(SKP2) and p27 were detected by real-time quantitative PCR (RT-qPCR) and Western blot methods. ［Results］ The proliferation of HeLa cells was inhibited by JQ1 in a time- and dose-dependent manner，a fifty percent inhibition of growth was seen after treated with 10μmol/L concentration of JQ1 for 72h. Compared with DMSO control group，the colony formation was inhibited significantly in 10μmol/L JQ1-treated group (the number of colony formation：3±2 vs 240±10，P<0.001)；and cell apoptosis was induced signiticantly by 10μmol/L JQ1 (the percentage of apoptosis：12.80±0.88 vs 2.90±0.27，P<0.01). Compared with DMSO control group，the expression of SKP2 was significantly decreased and the expression of p27 was significantly increased in JQ1-treated group (among them SKP2 mRNA expression folds：0.43±0.02 vs 1.00±0.03；p27 mRNA expression folds：2.60±0.13 vs 1.00±0.11，both with P<0.01). ［Conclusion］ JQ1 inhibits cell proliferation of HeLa cells via inducing apoptosis，which might depend on down-regulation of SKP2 and up-regulation of p27. |
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