| 冯同富,李 力,张 玮.利用基因芯片技术筛选人卵巢癌耐药差异表达基因[J].肿瘤学杂志,2016,22(7):527-532. |
| 利用基因芯片技术筛选人卵巢癌耐药差异表达基因 |
| Screening of Diffierential Expression Gene Related with Drug-resistance in Human Ovarain Cancer Cell Lines by Gene Chip |
| 投稿时间：2015-08-27 |
| DOI：10.11735/j.issn.1671-170X.2016.07.B001 |
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| 中文关键词: 卵巢肿瘤 铂类 耐药 基因芯片 |
| 英文关键词:ovarian neoplasms platinum drug resistance gene chip |
| 基金项目:广西壮族自治区大型仪器协作共用网资助项目 |
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| 摘要点击次数: 2042 |
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| 中文摘要: |
| 摘 要：［目的］ 比较卵巢上皮癌铂类耐药和非铂类耐药细胞间的基因表达差异，并进行相关基因的筛选和鉴定。［方法］ 采用人类全基因组表达谱芯片对卵巢癌铂类耐药细胞SKOV3/CB和亲本细胞SKOV3进行差异基因的筛选和分析；用半定量逆转录聚合酶联反应(RT-PCR)对部分芯片结果进行核对验证。［结果］ 共分离筛选出差异基因3506个，SKOV3/CB比SKOV3上调的差异基因共有1712个，其中上调10倍以上的共有163个，下调的差异基因共有1794个，其中下调10倍以上的共有70个。SKOV3/CB比SKOV3上调10倍以上的3个差异基因，即ZNF198、ERCC5和BIRC3经半定量RT-PCR验证，表明上述3个基因在耐药细胞株中的表达的确高于敏感细胞株。［结论］ 卵巢癌的多药耐药是一个多种基因参与的复杂过程，ZNF198、ERCC5和BIRC3参与卵巢癌铂类耐药。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the gene differential expression of platinum-resistance cell lines of ovarian epithelial cancer compared with the parent cell lines，and to screen and identify the associated genes. ［Methods］ Gene expression profiles were screened and analyzed by using whole genome oligonucleotide microarrays for carboplatin-sensitive ovarian cancer cell lines (SKOV3) and carboplatin-resistant cell lines(SKOV3/CB)，and the candidate genes which related to drug resistance were validated by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).［Results］ A total of 3506 differential genes were screened out，of which up- and down-regulated genes were 1712 and 1794 respectively in SKOV3/CB. And 163 genes and 70 genes were up-regulated more than 10 times in SKOV3/CB and SKOV3 respectively，which were related to many kinds of genes. And the expression levels of 3 genes，ZNF198，ERCC5 and BIRC3，selected from the more than 10 times in SKOV3/CB were verified by RT-PCR. ［Conclusion］ Many different kinds of genes are involved in the mechanism of mutidrug resistance. ZNF198，ERCC5 and BIRC3 are related to carboplatin-resistance in ovarian cancer. |
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