| 曹义娟,徐 惠,权 斌.5种药物对乳腺癌细胞分化和增殖抑制作用的比较[J].肿瘤学杂志,2016,22(6):452-456. |
| 5种药物对乳腺癌细胞分化和增殖抑制作用的比较 |
| Effects of Five Different Drugs on Differentiation Induction and Proliferation Inhibition of Breast Cancer Cell Line |
| 投稿时间：2015-11-19 |
| DOI：10.11735/j.issn.1671-170X.2016.06.B004 |
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| 中文关键词: 分化 MCF-7 细胞微环境 增殖抑制作用 |
| 英文关键词:differentiation MCF-7 microenvironment proliferation inhibition |
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| 摘要点击次数: 2042 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨全反式维甲酸(ATRA) 、1α，25-二羟维生素D3、盐酸强力霉素、雷洛芬和硫链丝菌肽对乳腺癌细胞MCF-7分化诱导、细胞微环境、细胞增殖影响的差异。［方法］ 油红O染色检测上述5种药物对MCF-7细胞分化诱导作用；台盼蓝染色检测上述5种药物对MCF-7细胞增殖的抑制作用。［结果］ 5种药物对MCF-7均有很强的分化诱导作用，且呈剂量依赖性。在0.08μmol/L时雷洛芬的分化诱导作用最强，盐酸强力霉素、硫链丝菌肽、1α，25-二羟维生素D3次之。诱导形成的小脂肪滴经油红O染色，显示相似颜色，但高浓度药物诱导形成的大脂肪块染色后，显示颜色的差异，ATRA诱导形成的大脂肪块染色后显示鲜红色，其他4种药物诱导形成的大脂肪块显示较暗的紫红色。脂肪块或脂肪滴主要位于细胞间质，少部分位于胞浆。硫链丝菌肽对MCF-7增殖的抑制作用远强于其他4种药物。［结论］ 5种药物均可诱导MCF-7细胞分化，改变细胞微环境。在0.08μmol/L时，雷洛芬的分化诱导率最高，盐酸强力霉素、硫链丝菌肽、1α，25-二羟维生素D3次之。它们所形成的脂肪滴成分可能相似，但有所差异。硫链丝菌肽对MCF-7增殖的抑制作用远强于其他4种药物。 |
| 英文摘要: |
| Abstract：［Objective］ To investigate the effects of different drugs on differentiation induction and proliferation inhibition of breast cancer MCF-7 cells. ［Methods］ Human breast cancer MCF-7 cells were treated with ATRA，calcitriol，doxycycline，raloxifene or thiostrepton in vitro，respectively. The differentiation induction of MCF-7 cells was detected by Oil Red O staining. The cell proliferation was measured by trypan blue assay. ［Results］ The differentiation of MCF-7 was induced by all 5 drugs in a dose-dependent manner. Among 5 different drugs，raloxifene was the most effective inducer at 0.08μmol/L，followed by doxycycline，thiostrepton and calcitriol. The induced lipid droplets showed similar color after Oil Red O staining. However，the lipid chunks accumulated by small lipid droplets induced by the above 5 different drugs at high concentrations showed different coloring after Oil Red O staining： the lipid chunks，induced by ATRA appeared to be bright red and the ones induced by other 4 drugs were dark purple. The lipid chunks or droplets were mainly located in the extracellular matrix，with few in cytoplasm. The proliferation inhibition of MCF-7 cells by thiostrepton was much stronger than that by other 4 drugs in a dose-dependent manner.［Conclusion］ ATRA，calcitriol，doxycycline，raloxifene or thiostrepton can induce the differentiation of breast cancer MCF-7 cells with various degrees. Among 5 drugs，thiostrepton has the strongest effect on the proliferation inhibition of MCF-7 cells. |
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