王时光,朱光辉,周联明.血清miR-194在结直肠癌诊断和预后判断中的价值研究[J].肿瘤学杂志,2016,22(5):360-364. |
血清miR-194在结直肠癌诊断和预后判断中的价值研究 |
Value of Serum MiR-194 in Diagnosis and Prognosis of Colorectal Cancer |
投稿时间:2015-12-09 |
DOI:10.11735/j.issn.1671-170X.2016.05.B005 |
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中文关键词: 结直肠肿瘤 血清 miR-194 诊断 预后 |
英文关键词:colorectal neoplasms serum miR-194 diagnosis prognosis |
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中文摘要: |
摘 要:[目的] 探讨血清miR-194在结直肠癌诊断和预后判断中的价值。[方法] 采用实时荧光定量PCR法检测60例结直肠癌患者和60例健康志愿者血清miR-194的相对表达水平,并分析miR-194表达与结直肠癌患者预后的关系。[结果] 结直肠癌患者的血清miR-194水平显著低于健康对照者(P<0.05)。按照血清miR-194相对定量值(临界值为1.015)进行结直肠癌诊断时的敏感性为70.0%,特异性为78.3%,阳性预测值为76.3%,阴性预测值为72.3%。血清miR-194可产生最大受试者工作特征曲线的曲线下面积(AUC)为0.86(95%CI:0.79~0.92)。N分期、cTNM分期、血清miR-194表达水平是结直肠癌患者预后的独立危险因素(P<0.05)。[结论] 血清miR-194可能是一种新的非侵入性指标用于结直肠癌患者的诊断及预后判断。 |
英文摘要: |
Abstract:[Objective] To assess the value of serum miR-194 in diagnosis and prognosis of colorectal cancer. [Methods] The relative expression level of serum miR-194 in 60 cases with colorectal cancer and 60 normal controls were detected by real-time fluorescence quantitative PCR assay,and the relationship between miR-194 expression and prognosis of colorectal cancer patients was analyzed. [Results] The level of serum miR-194 in patients with colorectal cancer was significantly lower than that of healthy controls(P<0.05). The sensitivity and specificity of miR-194(cut-off value as 1.015) for the diagnosis of colorectal cancer were 70.0% and 78.3%,and the positive predictive value and negative predictive value were 76.3% and 72.3%,respectively. The area under the ROC curve(AUC) was 0.86(95%CI:0.79~0.92). N staging,cTNM staging and serum miR-194 expression level were independent risk factors for prognosis of colorectal cancer(P<0.05). [Conclusion] Serum miR-194 might be a new non-invasive serum marker for the diagnosis and prognosis of colorectal cancer. |
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