| 王时光,朱光辉,周联明.血清miR-194在结直肠癌诊断和预后判断中的价值研究[J].肿瘤学杂志,2016,22(5):360-364. |
| 血清miR-194在结直肠癌诊断和预后判断中的价值研究 |
| Value of Serum MiR-194 in Diagnosis and Prognosis of Colorectal Cancer |
| 投稿时间：2015-12-09 |
| DOI：10.11735/j.issn.1671-170X.2016.05.B005 |
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| 中文关键词: 结直肠肿瘤 血清 miR-194 诊断 预后 |
| 英文关键词:colorectal neoplasms serum miR-194 diagnosis prognosis |
| 基金项目: |
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| 中文摘要: |
| 摘 要：［目的］ 探讨血清miR-194在结直肠癌诊断和预后判断中的价值。［方法］ 采用实时荧光定量PCR法检测60例结直肠癌患者和60例健康志愿者血清miR-194的相对表达水平，并分析miR-194表达与结直肠癌患者预后的关系。［结果］ 结直肠癌患者的血清miR-194水平显著低于健康对照者（P<0.05）。按照血清miR-194相对定量值(临界值为1.015)进行结直肠癌诊断时的敏感性为70.0％，特异性为78.3％，阳性预测值为76.3％，阴性预测值为72.3％。血清miR-194可产生最大受试者工作特征曲线的曲线下面积(AUC)为0.86（95%CI：0.79～0.92）。N分期、cTNM分期、血清miR-194表达水平是结直肠癌患者预后的独立危险因素（P<0.05）。［结论］ 血清miR-194可能是一种新的非侵入性指标用于结直肠癌患者的诊断及预后判断。 |
| 英文摘要: |
| Abstract：［Objective］ To assess the value of serum miR-194 in diagnosis and prognosis of colorectal cancer. ［Methods］ The relative expression level of serum miR-194 in 60 cases with colorectal cancer and 60 normal controls were detected by real-time fluorescence quantitative PCR assay，and the relationship between miR-194 expression and prognosis of colorectal cancer patients was analyzed. ［Results］ The level of serum miR-194 in patients with colorectal cancer was significantly lower than that of healthy controls（P<0.05）. The sensitivity and specificity of miR-194（cut-off value as 1.015） for the diagnosis of colorectal cancer were 70.0% and 78.3%，and the positive predictive value and negative predictive value were 76.3% and 72.3%，respectively. The area under the ROC curve（AUC） was 0.86（95%CI：0.79～0.92）. N staging，cTNM staging and serum miR-194 expression level were independent risk factors for prognosis of colorectal cancer（P<0.05）. ［Conclusion］ Serum miR-194 might be a new non-invasive serum marker for the diagnosis and prognosis of colorectal cancer. |
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