| 刘鲁迎,徐 琦,刘碧霞.不同剂量贝伐单抗对荷结肠癌细胞裸鼠移植瘤炎症因子表达的影响[J].肿瘤学杂志,2016,22(3):205-208. |
| 不同剂量贝伐单抗对荷结肠癌细胞裸鼠移植瘤炎症因子表达的影响 |
| Effect of Different Doses of Bevacizumab on Expressions of Inflammatory Factors in Xenografts from Nude Mice Bearing Human Colon Cancer Cells |
| 投稿时间：2015-12-03 |
| DOI：10.11735/j.issn.1671-170X.2016.03.B010 |
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| 中文关键词: 结肠肿瘤 贝伐单抗 裸鼠 炎症因子 |
| 英文关键词:colon neoplasms bevacizumab nude mice inflammatory factors |
| 基金项目:浙江省医药卫生优秀青年科技人才专项科研基金（2008QN004）；浙江省自然科学基金（LY14H160009） |
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| 中文摘要: |
| 摘 要：［目的］ 观察不同剂量贝伐单抗对肿瘤局部炎症因子表达的影响。［方法］ 接种人结肠癌DLD-1细胞的裸鼠24只，随机分为4组。对照组采用0.9%生理盐水，试验1组2.5mg/kg贝伐单抗，试验2组采用5mg/kg贝伐单抗，试验3组采用10mg/kg贝伐单抗，均分别于第1、5、9d注射。第10d处死裸鼠，留取皮下肿瘤。观察4组间肿瘤大小及肿瘤组织内炎症因子IL-1β、TNF-α、PGE-23 mRNA表达的差异。［结果］ 对照组及3个试验组间肿瘤体积比较差异无统计学意义。试验3组肿瘤组织内IL-1β mRNA表达的抑制最明显，与其他3组比较均有统计学差异（P=0.022、0.043、0.022）；试验3组肿瘤组织内TNF-α mRNA表达的抑制最明显，与对照组、试验2组比较差异有统计学意义（P=0.046、0.004）；PGE-23 mRNA表达与贝伐单抗的使用无相关性。［结论］ 10mg/kg贝伐单抗治疗后肿瘤组织内炎症指标IL-1β、TNF-α mRNA的表达明显下降，提示贝伐单抗能抑制肿瘤局部炎症反应。 |
| 英文摘要: |
| Abstract：［Purpose］ To observe the effect of different doses of bevacizumab on the expressions of local inflammatory factors in tumors. ［Methods］ DLD-1 human colon cancer cells were inoculated in 24 nude mice，and these mice were randomly divided into four groups：control group，experimental group 1，experimental group 2 and experimental group 3. The mice in these groups were treated with 0.9% normal saline，2.5mg/kg of bevacizumab，5mg/kg of bevacizumab and 10mg/kg of bevacizumab on day 1，5 and 9，respectively. The mice were sacrificed on day 10，and the subcutaneous xenografts were obtained. The differences in tumor sizes as well as IL-1β，TNF-α and PGE-23 mRNA expressions in tumors were investigated. ［Results］ No significant difference in tumor size was observed between the control group and the experimental groups. The IL-1β mRNA expression in experimental group 3 was significantly lower than that in the other three groups (control group，experimental group 1 and experimental group 2；P=0.022，0.043 and 0.022，respectively). The TNF-α mRNA expression in experimental group 3 was obviously inhibited，and its expression was statistically lower than that in the control group and experimental group 2(P=0.046，0.004). PGE-23 mRNA expression was not affected by bevacizumab treatment. ［Conclusion］ 10 mg/kg bevacizumab might significantly decrease IL-1β and TNF-α mRNA expressions in tumor tissue，which suggest that bevacizumab can inhibit local inflammatory reactions caused by tumors. |
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