| 黄显聪,赵 强,凌志强.EGFR mRNA上调在食管鳞状细胞癌患者预后预测中的意义[J].肿瘤学杂志,2016,22(3):199-204. |
| EGFR mRNA上调在食管鳞状细胞癌患者预后预测中的意义 |
| Significance of Up-regulation of EGFR mRNA in Prognosis for Esophageal Squamous Cell Carcinoma Patients |
| 投稿时间：2015-11-13 |
| DOI：10.11735/j.issn.1671-170X.2016.03.B009 |
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| 中文关键词: 食管肿瘤 鳞状细胞癌 表皮生长因子受体 基因突变 基因表达 预后 |
| 英文关键词:esophageal neoplasms squamous cell carcinoma epidermal growth factor receptor (EGFR) gene mutation gene expression prognosis |
| 基金项目:国家自然科学基金面上项目（81172081，81572822）；浙江省医药卫生科研计划项目（2012KYA029）；国家卫生计生委科学研究基金—浙江省医药卫生重大科技计划项目（WKJ-ZJ-1505）；浙江省胸部肿瘤（肺、食管）诊治技术研究重点实验室专项基金项目；浙江省卫生高层次创新人才培养工程项目；浙江省新世纪151人才工程重点资助项目 |
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| 中文摘要: |
| 摘 要：［目的］ 探讨表皮生长因子受体（EGFR）基因突变和表达在食管鳞状细胞癌中的意义。［方法］ 分别应用real-time RT-PCR、免疫组化技术检测105例食管鳞状细胞癌组织中EGFR mRNA及蛋白表达水平，应用ADx-ARMS和PCR-Sanger测序法检测上述标本中EGFR基因18、19、20、21外显子突变，分析EGFR表达状态与临床、病理特征及患者生存期的关系。［结果］ 105例食管鳞状细胞癌组织中仅3例（2.9%，3/105）呈EGFR蛋白弱阳性表达，与癌旁组织相比无显著性差异（P>0.05）。92.4%（97/105）的食管鳞状细胞癌组织中EGFR mRNA表达水平上调，与癌旁组织相比有显著性差异（P=0.000）。且肿瘤组织EGFR mRNA表达水平分别与淋巴结转移（P=0.001）、神经浸润（P=0.039）及TNM分期（P=0.003）显著相关。Kaplan-Meier生存分析显示，EGFR mRNA表达水平上调患者的平均生存期（16.7个月）显著短于EGFR mRNA表达正常/下调患者（19.1个月），其中8个EGFR mRNA正常表达病例生存情况均良好。多因素Cox回归分析表明，EGFR mRNA为食管鳞状细胞癌患者独立的预后因子，其相对危险度为1.368（P<0.001）。［结论］ EGFR mRNA可作为食管鳞状细胞癌患者预后标志物，其表达上调提示患者预后不良。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the significance of epidermal growth factor receptor (EGFR) gene mutation and expression in esophageal squamous cell carcinoma (ESCC). ［Methods］ Real-time RT-PCR and immunohistochemistry(IHC) technique were used respectively in testing the level of EGFR mRNA and protein expression in 105 cases with ESCC. Exon mutation of 18，19，20，21 in EGFR gene in the samples were detected by ADx-ARMS and PCR-Sanger sequencing. The relationship of the status of EGFR expression with clinico pathological parameters and survival in patients with ESCC was analyzed. ［Results］ Only 3 cases(2.9%，3/105) had weak positive expression of EGFR protein among 105 cases with ESCC，which had no significant difference compared with cancer adjacent tissues (P>0.05). While there was 92.4% (97/105) ESCC tissues with over-expression of EGFR mRNA which had significant difference compared with cancer adjacent tissues (P=0.000). The expression of EGFR mRNA in ESCC significantly associated with lymph node metastasis (P=0.001)，neural invasion (P=0.039) and TNM stage (P=0.003). Kaplan-Meier survival analysis showed that the mean survival of patients with high expression of EGFR mRNA(16.7 months) was significantly shorter than that in patients with normal/low expression (19.1 months)，and 8 cases among the patients with normal expression of EGFR mRNA were still alive. The multivariate Cox regression analysis demonstrated that EGFR mRNA expression was an independent prognostic factor for survival，with RR of 1.368(P<0.001). ［Conclusions］ EGFR mRNA might be used as a prognostic marker in patients with ESCC，and its up-regulation indicates poor prognosis for ESCC patients. |
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