| 陈忠坚,倪茂巍,毛伟敏.恶性间皮瘤高迁移率蛋白1乙酰化修饰分析[J].肿瘤学杂志,2016,22(3):194-198. |
| 恶性间皮瘤高迁移率蛋白1乙酰化修饰分析 |
| Acetylated Modifications of High Mobility Group Box-1 (HMGB1) in Malignant Mesothelioma |
| 投稿时间：2016-03-06 |
| DOI：10.11735/j.issn.1671-170X.2016.03.B008 |
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| 中文关键词: 恶性间皮瘤 高迁移率蛋白1 乙酰化 质谱 |
| 英文关键词:malignant mesothelioma high mobility group box-1 acetylation mass spectrometry |
| 基金项目:浙江省分析平台项目；浙江省肿瘤医院1022人才计划资助 |
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| 中文摘要: |
| 摘 要：［目的］ 探索恶性间皮瘤中高迁移率蛋白1（HMGB1）乙酰化修饰位点信息。［方法］利用Q Exactive四极杆—静电场轨道阱高分辨质谱和Proteome Discoverer软件，研究恶性间皮瘤细胞NCI-H2452中HMGB1的乙酰化位点，以肺癌细胞A549和卵巢癌细胞A2780作为参照。［结果］ 恶性间皮瘤细胞中HMGB1的较多Lysine发生乙酰化修饰，包括K43、K50、K57、K59、K60、K90、K136、K137、K167、K170，除K43和K90外，均为新报道的HMGB1乙酰化位点；然而在肺癌细胞和卵巢癌细胞中均未检测到HMGB1乙酰化修饰。［结论］ 本研究发现恶性间皮瘤中HMGB1存在特异性的乙酰化位点，其可能是潜在恶性间皮瘤诊断分子标志物。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the characteristics of acetylated modification of high mobility group box-1 (HMGB1) in malignant mesothelioma.［Methods］ Malignant mesothelioma cells NCI-H2452 and two other cancer cells as controls(lung cancer cell lines A549 and ovarian cancer cell lines A2780) were extracted of total proteins. Then，the proteins were prepared and analyzed by Easy-nano LC1000 combined with Q Exactive mass spectrometry. Acetylation sites were obtained using Proteome Discoverer software. ［Results］ HMGB1 from malignant mesothelioma cells contained lots of lysine acetylated，including K43，K50，K57，K59，K60，K90，K136，K137，K167 and K170. They were novel acetylation sites of HMGB1 except for K43 and K90. However，there were no acetylated modifications of HMGB1 detected in the lung and ovarian cancer cells. ［Conclusion］ Acetylated modification might be characteristic of malignant mesothelioma，and the acetylation sites in our study might be diagnostic markers for malignant mesothelioma. |
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