| 徐志远,程向东,杜义安.槐耳浸膏逆转人胃癌耐药细胞多药耐药性的实验研究[J].肿瘤学杂志,2016,22(2):129-133. |
| 槐耳浸膏逆转人胃癌耐药细胞多药耐药性的实验研究 |
| An Experimental Study of the Huaier Role in Reversing Multidrug Resistance of Chemo-resistant Gastric Cancer Cells |
| 投稿时间：2015-11-25 |
| DOI：10.11735/j.issn.1671-170X.2016.02.B011 |
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| 中文关键词: 胃肿瘤；化疗耐药；GSH 槐耳 |
| 英文关键词:gastric neoplasms chemoresistance GSH Huaier |
| 基金项目:浙江省中医药重点研究计划（2012ZZ002）；浙江省中医药管理局一般项目（2013ZA022，2015ZA090） |
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| 中文摘要: |
| 摘 要：［目的］ 体外观察槐耳浸膏对胃癌耐药细胞（SGC-7901/FU）多药耐药性的影响。［方法］ CCK-8法检测SGC7901/FU细胞的耐药性；谷胱甘肽（GSH）还原酶循环法检测SGC-7901/FU细胞内GSH含量的变化；流式细胞仪检测SGC-7901/FU细胞的凋亡率。［结果］ SGC-7901/FU细胞内GSH含量［(13.20±1.51) nmol/106细胞］较SGC7901细胞［(6.47±0.85) nmol/106细胞］显著增高(P＜0.05)；槐耳浸膏能抑制SGC7901/FU细胞增殖，24h、48h的半数抑制浓度(IC50)分别为2.43mg/ml和2.04mg/ml； 0.1~0.5mg/ml浓度范围内槐耳浸膏增加SGC-7901/FU细胞对5-Fu、DDP的敏感性，并降低GSH含量；槐耳浸膏与5-Fu联用能显著增加SGC-7901/FU细胞凋亡率 (P＜0.05)。［结论］ 槐耳浸膏能部分逆转SGC-7901/FU细胞的耐药性，其机制可能是通过抑制细胞的GSH合成、诱导细胞凋亡而实现的。 |
| 英文摘要: |
| Abstract：［Purpose］To investigate the effect of huaier on the multidrug resistance(MDR) transformation of chemo-resistant gastric cancer cells(SGC-7901/FU)．［Methods］ CCK-8 method was used to calculate the 50% inhibitory concentration(IC50) of SGC-7901/FU cells. glutathione reductase recycling assay was used to determine the GSH content of SGC-7901/FU cells. Flow cytometry was used to determine the apoptosis rate of SGC-7901/FU cells. ［Results］ The level of GSH in SGC-7901/FU cells［(13.20±1.51)nmol/106 cells］ was significantly higher than that in SGC-7901 cells［(6.47±0.85)nmol/106 cells］(P＜0.05). Huaier could inhibit the proliferation of SGC-7901/FU cells，the IC50 value was 2.43mg/ml and 2.04mg/ml，respectively，after 24h and 48h exposure. The combined used of Huaier，at 0.1~0.5mg/ml concentration，could increase the GSH content and the chemo-sensitivity of SGC-7901/FU cells to DDP and 5-Fu. The value of apoptosis rate increased significantly through simutaneous use of Huaier and 5-Fu(P＜0.05). ［Conclusion］ Huaier could partly reverse the chemoresistance of SGC-7901/FU cells，and the underline mechanism might include inhibition of GSH sythesis and induction of apoptosis. |
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