| 龙洪清,向 赟,张 振.HPV16 E6对Hippo途径效应分子YAP表达和定位的影响[J].肿瘤学杂志,2016,22(2):125-128. |
| HPV16 E6对Hippo途径效应分子YAP表达和定位的影响 |
| Effect of HPV16 E6 Protein on the Expression and Localization of Effector YAP in the Hippo Pathway |
| 投稿时间：2015-10-25 |
| DOI：10.11735/j.issn.1671-170X.2016.02.B010 |
|
 |
| 中文关键词: 人乳头瘤病毒 E6蛋白 Hippo信号途径 YAP |
| 英文关键词:human papillomavirus E6 protein Hippo signal pathway Yes-associated protein |
| 基金项目:湖北省卫生厅科研一般项目（JX6B97） |
|
| 摘要点击次数: 2186 |
| 全文下载次数: 740 |
| 中文摘要: |
| 摘 要：［目的］ 研究HPV16 E6和Hippo途径效应分子YAP的相关性。［方法］ 用Western Blot、RT-PCR和激光共聚焦显微镜观察HPV16 E6过表达或敲减后YAP的表达和定位，以及其直接作用的下游基因结缔组织生长因子（CTGF）的表达。［结果］ HPV16 E6敲减或过表达时YAP相应地表达减少或增加，磷酸化的YAP增加或减少，YAP主要定位于胞浆或胞浆和胞核中，YAP下游CTGF的表达相对应减少或增加。［结论］ HPV16 E6诱导Hippo途径失常，YAP的表达升高，可能提升HPV16 E6的致癌性，Hippo途径可能是治疗高危性HPV感染相关疾病的候选药物作用靶标。 |
| 英文摘要: |
| Abstract：［Purpose］ To explore the association between HPV16 E6 and Yap. ［Methods］ The expression and localization of YAP and its downstream connective tissue growth factor (CTGF) were analyzed using Western Blot，RT-PCR and laser scanning confocal microscope after HPV16 E6 overexpression and knockdown.［Results］ HPV16 E6 knockdown or overexpression induced the decreasing or increasing YAP expression，and Yap mainly located in cytoplasm or cytoplasm and nuclear，and CTGF expression decreased or increased.［Conclusion］ HPV16 E6 induces Hippo pathway deregulation and Yap overexpression might promote HPV16 E6 pathogenesis，implying that Hippo pathway might be candidate drug target of hr-HPV infection-induced diseases. |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |