| 龚虹云,宋启斌,胡伟国.GNAS1 T393C多态性与放射性肺炎的相关性研究[J].肿瘤学杂志,2016,22(2):93-98. |
| GNAS1 T393C多态性与放射性肺炎的相关性研究 |
| Association of Single Nucleotide Polymorphisms of GNAS1 T393C with Radiation Pneumonitis |
| 投稿时间：2015-10-06 |
| DOI：10.11735/j.issn.1671-170X.2016.02.B004 |
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| 中文关键词: GNAS1 T393C 单核苷酸多态性 肺肿瘤 放射治疗 不良反应 |
| 英文关键词:GNAS1 T393C SNP lung neoplasms radiotherapy side effects |
| 基金项目:国家自然科学基金资助（81372407） |
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| 中文摘要: |
| 摘 要：［目的］ 分析GNAS1 T393C多态性与肺癌放疗疗效、血液毒性、放射性食管炎和放射性肺炎的相关性。［方法］ 共收集经病理证实的接受放疗的Ⅲ~Ⅳ期肺癌患者67例。应用PCR扩增目的基因片段，sanger双脱氧测序方法分析GNAS1 T393C位点单核酸多态性的基因型。应用卡方检验和Logistic回归分析临床因素及基因型与肺癌放疗疗效、血液毒性、放射性食管炎和放射性肺炎的相关性。［结果］ 本组研究病例中位年龄为61岁。单因素分析显示同时使用化疗可以增加肺癌放疗疗效（P=0.045），但是相应的骨髓抑制发生率增加（P=0.004）。肺鳞癌患者更容易发生放射性肺炎（P=0.015）。GNAS1 T393C等位基因T携带者发生放射性肺炎的比例明显高于等位基因C携带者（P=0.035）；进一步通过Logistic回归分析校正其他可能临床因素影响后，提示GNAS1 TT基因型发生放射性肺炎的可能性更大（P=0.06，OR=0.26，95%CI：0.06~1.09）。［结论］ GNAS1 T393C等位基因T与放射性肺炎相关，可能成为其预测因素，需要扩大样本量进行更深入的研究。 |
| 英文摘要: |
| Abstract：［Purpose］ To examine the possible relationship between SNP of GNAS1 T393C and curative effect of radiotherapy and radiation-induced hematological toxicity，esophagitis and pneumonia.［Methods］ Genomic DNA was extracted from whole blood of 65 advanced lung cancer patients underwent radiotherapy. Allelic discrimination of GNAS1 was performed by quantitative real-time polymerase chain reaction. Genetyping was correlated with curative effect of radiotherapy and radiation-induced hematological toxicity，esophagitis and pneumonia. The endpoint was grade≥3 hematological toxicity and grade≥2 radiation-induced esophagitis and pneumonia. The Chi-square criterion method and Logistic regression analysis were used to assess the significance of the clinical factors and genotypes for curative effect of radiotherapy and radiation-induced hematological toxicity，esophagitis and pneumonitis. ［Results］ Median age was 61 years old (ranged 35 to 80 years). At univariate analysis，chemotherapy could improve the efficacy of radiotherapy in lung cancer (P=0.045)，but increased the hematotoxicity (P=0.004). Patients with squanae type and allele T carrier in GNAS1 T393C gene had more higher incidence of radiation pneumonitis(P=0.015，P=0.035).［Conclusions］ Genetic polymorphism in the GNAS1 T393C might relate to radiation pneumonitis，which may be a prognostic factor. The results need large sample research to identify. |
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