| 吴其肯,刘玉君,王海江.ERβ基因表达与肝细胞肝癌术后复发的相关性及机制研究[J].肿瘤学杂志,2015,21(10):835-838. |
| ERβ基因表达与肝细胞肝癌术后复发的相关性及机制研究 |
| Study of Correlation and Mechanism Between ERβ Expression and Postoperative Recurrence of Hepatocellular Carcinoma |
| 投稿时间：2015-03-25 |
| DOI：10.11735/j.issn.1671-170X.2015.10.B011 |
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| 中文关键词: ERβ 肝肿瘤 术后复发 分子标志物 |
| 英文关键词:ERβ liver neoplasms postoperative recurrence biomarker |
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| 摘要点击次数: 1985 |
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| 中文摘要: |
| 摘 要：［目的］ 研究雌激素受体2 (ERβ)与肝细胞肝癌 (HCC)术后复发的相关性以及其潜在分子机制。［方法］ 通过qRT-PCR技术与Western Blot检测ERβ在HCC术后两年内复发的8例患者以及10例未复发患者癌组织样本内的表达差异。同时，利用三种交叉验证实验 (留一交叉验证、十折交叉验证和蒙特卡罗交叉验证)分析ERβ表达对HCC样本的术后复发预后的分型准确性。此外，进一步预测ERβ下游调控基因，分析下游调控基因显著参与的生物学功能以及信号通路，探究其可能的分子机制。［结果］ ERβ基因在术后复发的HCC样本中显著高表达，与术后复发明显相关，且通过交叉验证实验结果发现ERβ对HCC样本有较好的分型准确性，三种验证方法的分型准确率分别为75% (73%~76%)、73% (72%~73%)与71% (71%~72%)。最后通过对ERβ下游调控基因的功能富集分析发现，ERβ显著参与到细胞增殖、RNA转录等HCC术后发展相关的功能与通路中。［结论］ ERβ具有良好的HCC术后复发预测效能，具有成为HCC术后复发分子标志物的潜力，具有一定的研究价值。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the correlation and mechanism between estrogen receptor 2 (ERβ) expression and postoperative recurrence of hepatocellular carcinoma(HCC). ［Methods］ The expression of ERβ between 8 patients with postoperative recurrence of HCC and 10 patients with no recurrence of HCC was detected by qRT-PCR and Western Blot. The classified power of ERβ was analyzed by three cross-validation algorithms［leave-one-out cross validation (LOOCV)，10-fold cross validation (10-fold CV) and Montecarlo cross validation (MCCV)］. Moreover，the downstream regulated genes of ERβ were predicted. Then，biological functional enrichment analysis was used to detect the functions and processes related to these regulated genes.［Results］ The expression of ERβ gene was higher in patients with postoperative recurrence of HCC. ERβ had a high prediction accuracy for classified HCC ［LOOCV：75% (73%~76%)，10-fold CV：73% (72%~73%)，MCCV：71% (71%~72%)］. At last，functional enrichment analysis showed that the regulated genes of ERβ were significantly involving in functions of cell proliferation，apoptosis，differentiation，angiogenesis and HCC progression related functions and pathways. ［Conclusion］ ERβ shows a good classified power for recurrence of HCC，and those may be candidate biomarkers of postoperative recurrence of HCC and worth researching. |
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