| 赵 球,郭人花.Akt及Survivin表达在非小细胞肺癌中的临床意义[J].肿瘤学杂志,2015,21(9):757-761. |
| Akt及Survivin表达在非小细胞肺癌中的临床意义 |
| The Clinical Significance of Expressions of Akt and Survivin in Non-small Cell Lung Cancer |
| 投稿时间：2014-12-08 |
| DOI：10.11735/j.issn.1671-170X.2015.09.B012 |
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| 中文关键词: 非小细胞肺癌 Akt Survivin 肿瘤转移 |
| 英文关键词:non-small cell lung cancer(NSCLC) Akt Survivin neoplasm metastasis |
| 基金项目:国家自然科学基金(81172217) |
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| 中文摘要: |
| 摘 要：［目的］ 探讨Akt及Survivin表达在非小细胞肺癌(NSCLC)中的临床意义。 ［方法］ 采用qRT-PCR技术及Western blot法检测55例NSCLC原发灶、癌旁及正常组织中Akt及Survivin的表达。［结果］ qRT-PCR和Western blot均显示Akt及Survivin在NSCLC原发灶中的表达明显高于癌旁及正常组织（P均<0.05）；qRT-PCR显示在术后1年内发生远处转移的患者组织中的表达明显高于其在没有发生远处转移的组织中的表达（P=0.0297），在有淋巴结转移的NSCLC原发灶中的表达明显高于无淋巴结转移者（P=0.0137）。Akt及Survivin在分级为Ⅱ/Ⅲ级的NSCLC中的表达均明显高于在Ⅰ级患者中表达（P=0.0487，P=0.0467），但Akt及Survivin的表达与患者的年龄、性别、肿瘤的大小、分化程度及分期都没有明显关系（P>0.05）。两种方法检测均显示Akt与Survivin的表达成正相关(r=0.5545，P<0.05；r=0.6144，P<0.05 )。［结论］ Akt和Survivin相互关联，可能协同促进NSCLC的发生发展及侵袭转移。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the clinical significance of expressions of Akt and Survivin in non-small cell lung cancer(NSCLC). ［Methods］ The expressions of Akt and Survivin were detected in cancer tissues，cancer adjacent tissues and normal tissues in samples from 55 cases with NSCLC by qRT-PCR and Western blot methods. ［Results］ Akt and Survivin expressions were significantly higher in cancer tissues than those in cancer adjacent tissues and normal tissues by both qRT-PCR and Western blot methods（P<0.05 ). qRT-PCR detection showed that the level of Survivin was higher in patients with distant metastasis within one year postoperation than that in patients without distant metastasis(P=0.0297)，and also was higher in patients with lymph metastasis than that in patients without lymph metastasis(P=0.0137). Akt and Survivin were higher in NSCLC patients with grade Ⅱ/Ⅲ than those in patients with grade Ⅱ（P=0.0487，P=0.0467），but both of them had no significant correlation with patients’age，gender，tumor size，differentiation and staging(P>0.05). Both qRT-PCR and Western blot detection showed that Akt and Survivin were positively correlated (r=0.5545，P<0.05；r=0.6144，P<0.05). ［Conclusion］ Akt and Survivin are correlated，which might have synergistic effect on development and invasion and metastasis of NSCLC. |
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