徐 驰,汪 栋,苏长青.miRNA-145抑制肺癌细胞系A549迁移侵袭的研究[J].肿瘤学杂志,2015,21(9):742-747. |
miRNA-145抑制肺癌细胞系A549迁移侵袭的研究 |
Inhibitory Effect of MicroRNA-145 on Migration and Invasion of Human Lung Cancer Cell Line A549 |
投稿时间:2015-06-02 |
DOI:10.11735/j.issn.1671-170X.2015.09.B009 |
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中文关键词: 肺肿瘤 miRNA-145 细胞迁移 肿瘤浸润 细胞增殖 |
英文关键词:lung neoplasms miRNA-145 cell migration neoplasm invasion cell proliferation |
基金项目:军队临床高新技术重大项目(2010gxjs027);福建省科技计划项目(2012D021);福建省自然科学基金(2015J01486) |
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中文摘要: |
摘 要:[目的] 观察miRNA-145对肺癌细胞系A549迁移、侵袭和克隆形成的影响,探讨其潜在的应用价值。[方法] 采用miRNA-145过表达的慢病毒载体上调A549细胞中miRNA-145的表达量,Transwell迁移及侵袭实验分析miRNA-145表达上调前后A549细胞的体外迁移、侵袭能力,平板克隆形成实验分析miRNA-145表达上调前后A549细胞的体外增殖能力。[结果] RT-PCR结果表明,侵染了miRNA-145过表达载体的A549细胞中miRNA-145水平明显高于阴性对照组及空白对照组,差异有统计学意义(P<0.01);Transwell迁移和侵袭实验结果表明,过表达miRNA-145的A549细胞过膜数少于阴性对照及空白对照组,差异有统计学意义(P<0.01);平板克隆形成实验结果表明,过表达miRNA-145的A549细胞平板克隆形成能力明显降低,差异有统计学意义(P<0.01)。[结论] miRNA-145的过表达能够抑制肺癌细胞系A549的迁移、侵袭和克隆形成,由此提示miRNA-145有可能成为肺癌治疗的潜在靶点。 |
英文摘要: |
Abstract:[Purpose] To observe the effect of miRNA-145 on the migration and invasion and proliferation abilities of human lung cancer cell line A549,and to discuss its potential application. [Methods] Hsa-miR-145-5p was used to increase miRNA-145 expression in A549 cell lines,then the migration ability of A549 cell was observed by Transwell migration assay before and after treatment with hsa-miR-145-5p,the invasive abilitiy of A549 cell was observed by Transwell invasion assay before and after treatment with hsa-miR-145-5p,the proliferation ability of A549 cell was observed by plate clone formation assay before and after treatment with hsa-miR-145-5p. [Results] The result of real-time RT-PCR showed that miRNA-145 expression in A549 cell infected by hsa-miR-145-5p was higher than others(P<0.01). Up-expression of miRNA-145 in A549 cell inhibited the migration and invasion and proliferation abilities of A549 cells(P<0.01). [Conclusion] Up-expression of miRNA-145 plays an inhibitory role in the migration and invasion and proliferation of human lung cancer cell line A549,which indicate that it might be a new target for biotherapy of lung cancer. |
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