| 方 鹏,邹 甜,江晓肖.白花蛇舌草注射液对鼻咽癌CNE-2细胞体外增殖抑制及凋亡作用的研究[J].肿瘤学杂志,2015,21(9):737-741. |
| 白花蛇舌草注射液对鼻咽癌CNE-2细胞体外增殖抑制及凋亡作用的研究 |
| Effect of Hedyotis Diffusa on Proliferation and Apoptosis of Nasopharyngeal Carcinoma CNE-2 Cells in vitro |
| 投稿时间：2015-03-16 |
| DOI：10.11735/j.issn.1671-170X.2015.09.B008 |
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| 中文关键词: 白花蛇舌草 鼻咽肿瘤 细胞凋亡 |
| 英文关键词:Hedyotis diffusa nasopharyngeal neoplasms cell apoptosis |
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| 中文摘要: |
| 摘 要：［目的］ 探讨白花蛇舌草注射液对鼻咽癌CNE-2细胞体外增殖抑制作用及凋亡机制。［方法］ MTT法检测白花蛇舌草注射液对鼻咽癌CNE-2细胞的抑制率，Annexin-V-FITC/PI 双染法和Hoechst法检测细胞凋亡，Western blot法检测Bcl-2、Survivin、Bax、Caspase-3蛋白表达。［结果］ 不同浓度白花蛇舌草注射液对鼻咽癌CNE-2细胞具有抑制增殖作用，呈时间浓度依赖性（P＜0.05）。8ml/L白花蛇舌草注射液能明显诱导CNE-2细胞凋亡，与对照组相比，具有统计学意义（P＜0.05）。8ml/L白花蛇舌草注射液能诱导CNE-2细胞Bcl-2、Survivin蛋白表达降低、Bax及Caspase-3蛋白表达增加。［结论］ 白花蛇舌草注射液对鼻咽癌CNE-2细胞具有明显的体外增殖抑制作用，且呈时间浓度依赖性，其机制可能与诱导细胞凋亡，降低Bcl-2、Survivin蛋白表达，增加Bax及Caspase-3蛋白表达有关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the anti-proliferation and apoptosis effects of Hedyotis diffusa in nasopharyngeal carcinoma CNE-2 cells in vitro and the mechanisms.［Methods］ The inhibition effect was detected by MTT method. Apoptosis was detected by Annexin-V-FITC/PI and Hoechst. Bcl-2，Survivin，Bax and Caspase-3 expressions were detected by Western blot assay.［Results］ The growth of CNE-2 cells was inhibited by Hedyotis diffusa in a time and dose manner（P＜0.05）. Cell apoptosis was induced significantly by 8ml/L Hedyotis diffusa compare to the controls（P＜0.05）. The Bcl-2 and Survivin proteins’ expressions were decreased，and the Bax and Caspase-3 proteins’ expressions were increased by 8ml/L Hedyotis diffusa. ［Conclusion］ Hedyotis diffusa might inhibit proliferation and induce apoptosis of CNE-2 cells in a time and dose manner，and the mechanism may be related to decreasing the levels of Bcl-2 and Survivin proteins and increasing the levels of Bax and caspase-3 proteins. |
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