| 焦德敏,胡慧珍,唐夏莉.Nectin 4在肺癌组织中的表达及干扰nectin 4 对肺癌细胞增殖和迁移侵袭的影响[J].肿瘤学杂志,2015,21(8):633-639. |
| Nectin 4在肺癌组织中的表达及干扰nectin 4 对肺癌细胞增殖和迁移侵袭的影响 |
| Expression of Nectin 4 in Lung Cancer Tissues and the Effect of Nectin 4 Silence on Lung Cancer Cell Proliferation，Migration and Invasion |
| 投稿时间：2014-09-28 |
| DOI：10.11735/j.issn.1671-170X.2015.08.B002 |
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| 中文关键词: nectin 4 肺肿瘤 侵袭 迁移 |
| 英文关键词:nectin 4 lung neoplasms migration invasion |
| 基金项目:浙江省医药卫生科技计划项目（2011KYA139) |
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| 中文摘要: |
| 摘 要：［目的］ 研究nectin 4在肺癌组织中的表达及其在肺癌细胞增殖、迁移侵袭过程中的作用。［方法］ 免疫组化法检测20例肺癌组织及其对应癌旁组织中nectin 4的表达情况；QPCR及Western Blot分别检测不同肺癌细胞株及正常肺上皮细胞中nectin 4 mRNA和蛋白的表达情况；设计nectin 4干扰 RNA序列，转染肺癌细胞后，MTT法检测细胞的增殖能力。划痕愈合实验和Transwell小室法分别检测细胞的迁移能力和侵袭能力。［结果］ 免疫组化结果显示nectin 4在癌旁组织中不表达，而40%的肺癌组织中表达阳性（8/20），且nectin 4 的表达与肺癌患者淋巴结转移明显相关（r=0.612，P=0.004）。QPCR及Western Blot结果显示，正常肺上皮细胞BEAS-2B中nectin 4表达较低，而在A549和PC-9肺癌细胞株中nectin 4表达明显较高。 设计的其中一条nectin 4 siRNA可以降低肺癌A549和PC-9细胞nectin 4蛋白表达70%以上，且nectin 4基因干扰后，肺癌细胞的增殖能力减弱，迁移和侵袭能力也受到明显抑制。［结论］ Nectin 4表达水平与肺癌细胞的增殖、迁移和侵袭密切相关，可以作为肺癌转移治疗的重要靶点。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the expression of nectin 4 in lung cancer tissues and evaluate the effect of nectin 4 on lung cancer cell proliferation，migration and invasion.［Methods］ Immunohistochemistry was used to detect the protein expression of nectin 4 in 20 lung cancer tissues and corresponding normal tissues. QPCR and Western Blot were respectively used to detect mRNA and protein expression of nectin 4 in different kinds of lung cancer cells and normal lung epithelial cells. Synthetic siRNA duplex was applied to knock down nectin 4 gene in lung cancer cells. The cell proliferation was measured by MTT assay，and the cell migration and invasion abilities were accessed by wound healing and transwell assays，respectively.［Results］ Immunohistochemical results showed that nectin 4 protein expression was positive in 40% (8/20) lung cancer tissues，while negative in all detected normal lung tissues. Moreover，nectin 4 expression closely correlated with lymph node metastasis（r=0.612，P=0.004). QPCR and Western Blot results showed that nectin 4 was high expression in A549 and PC-9 lung cancer cells and low expression in BEAS-2B normal lung epithelial cells at both the mRNA and protein levels. Expressing siRNA2 significantly reduced the nectin 4 expression in A549 and PC-9 cells，and the growth，migration and invasion of the two lung cancer cells were also obviously inhibited.［Conclusion］ The nectin 4 expression level relates with proliferation，migration and invasion of lung cancer cells. Nectin 4 might be a target gene for treatment of lung cancer metastases. |
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