| 范立侨,李 勇,李巧霞.塞来昔布联合氟尿嘧啶对MFC细胞615小鼠原位移植瘤COX-2、Fas表达的影响及意义[J].肿瘤学杂志,2015,21(5):355-359. |
| 塞来昔布联合氟尿嘧啶对MFC细胞615小鼠原位移植瘤COX-2、Fas表达的影响及意义 |
| Effect of Celecoxib Combined with Fluorouracil on Expressions of COX-2，Fas Proteins in 615 Mice Orthotopic Transplantation Tumors of MFC Cells and Their Significance |
| 投稿时间：2015-03-10 |
| DOI：10.11735/j.issn.1671-170X.2015.05.B001 |
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| 中文关键词: 胃肿瘤 环氧合酶-2 氟尿嘧啶 Fas蛋白 |
| 英文关键词:gastric neoplasms cyclooxygenase-2 fluorouracil Fas protein |
| 基金项目:国家自然科学基金(81372580)；河北省自然科学基金(C2011206086) ；河北省卫生厅医学科学研究重点课题（20110456） |
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| 中文摘要: |
| 摘 要：［目的］ 通过测定在塞来昔布及氟尿嘧啶干预下的小鼠前胃癌细胞（MFC）615小鼠原位移植瘤中环氧合酶（COX-2）、Fas的表达，对COX-2抑制剂联合氟尿嘧啶对胃癌的影响及意义进行探讨。［方法］ 制备MFC细胞615小鼠原位移植胃癌模型。造模成功后分为塞来昔布组、联合用药组及对照组，其中塞来昔布25mg/kg灌胃，氟尿嘧啶50mg/kg腹腔注射。观察各组小鼠一般情况。干预3周后处死小鼠，计算抑瘤率。SP免疫组织化学法检测各组肿瘤COX-2、Fas 表达。［结果］ 造模成功率100.00%（30/30）。联合用药组、塞来昔布组的小鼠一般情况优于对照组。实验结束后3组比较，塞来昔布组、联合用药组比对照组肿瘤体积和瘤重的抑制率均明显升高（P＜0.05）。与塞来昔布组相比，联合用药组肿瘤体积更小、瘤重减轻（P<0.05）。COX-2蛋白在塞来昔布组、联合用药组表达阳性率明显低于对照组，而Fas 蛋白的表达则明显高于对照组（P<0.05）。［结论］ 塞来昔布及氟尿嘧啶对胃癌有抑制作用，其机制与调节COX-2、Fas蛋白表达有关。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of celecoxib combined with fluorouracil on the expressions of COX-2，Fas proteins in 615 mice orthotopic transplantation tumors of MFC cells and to investigate the influence of COX-2 inhibitor or combined with fluorouracil on gastric cancer. ［Methods］ 615 mice MFC orthotopic transplantation models were established. The mice were divided into celecoxib group(celecoxib 25mg/kg orally)，combination group (celecoxib 25mg/kg orally and fluorouracil 50mg/kg intraperitoneal injection)，and control group. General data of mice were recorded. Models were sacrificed after 3 weeks with intervention，and inhibition rate of tumor was analyzed. Expressions of COX-2，Fas proteins were determined with SP immunohistochemical method. ［Results］ All models were established (100.00%，30/30). General situation of celecoxib group and combination group was better than that of control group. After intervention，the inhibition of tumor volume and weight were higher in celecoxib group and combination group than those in control group(P<0.05). Compared with celecoxib group，tumor volume and weight were lower in combination group(P<0.05). Positive rate of COX-2 protein expression was lower in celecoxib group and combination group than that in control group，whereas positive rate of Fas protein expression was higher in celecoxib group and combination group than that in control group(P<0.05). ［Conclusion］ Celecoxib combined with fluorouracil could inhibit gastric cancer by regulating COX-2，Fas proteins. |
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