| 孙宏伟,王 飞,王荣福.放射性碘标记热休克蛋白90α单克隆抗体用于肿瘤放射免疫治疗的研究[J].肿瘤学杂志,2015,21(4):270-274. |
| 放射性碘标记热休克蛋白90α单克隆抗体用于肿瘤放射免疫治疗的研究 |
| Study on Tumor Radioimmunotherapy Using Radioiodinated Labeled Heat Shock Protein 90α Monoclonal Antibody |
| 投稿时间：2015-02-05 |
| DOI：10.11735/j.issn.1671-170X.2015.04.B003 |
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| 中文关键词: 热休克蛋白90α 肿瘤 单克隆抗体 放免治疗 131I |
| 英文关键词:Hsp90α-mAb tumor monoclonal antibody radioimmunotherapy 131I |
| 基金项目:国家重大科学仪器设备开发专项（2011YQ03011409）、 |
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| 中文摘要: |
| 摘 要：［目的］ 用放射性碘Na131I标记热休克蛋白90α单克隆抗体，研究其在胶质瘤荷瘤小鼠模型的生物分布以及放射免疫治疗效果。［方法］ 热休克蛋白90α单克隆抗体的放射性131I标记采用氯胺T法进行。标记抗体经尾静脉注射到U87细胞荷瘤小鼠模型体内，在注射体内后6、12、24h处死小鼠，测量其在各组织器官内的分布。放免实验分为实验组、对照组和未标记组，每组6只小鼠，注射药物后每5天测量肿瘤体积，连续40天。并记录全部小鼠注射后的生存时间。［结果］ 热休克蛋白90α单克隆抗体标记效率达到56%，纯化后放射化学纯度超过95%。分布数据表明131I-Hsp90α单克隆抗体主要累积在肝脏、脾脏和肿瘤。治疗组肿瘤体积明显小于其他两组，抑瘤率高于其余两组且生存时间更长。［结论］ 热休克蛋白90α单克隆抗体可以用氯胺T法成功标记131I。131I标记热休克蛋白90α单克隆抗体可以被U87荷瘤小鼠的肿瘤组织特异性摄取，其用于放免治疗有一定的研究价值和临床应用前景。 |
| 英文摘要: |
| Abstract：［Purpose］ We labeled Hsp90α monoclonal antibody with radioiodine Na131I，investigated its biodistribution and potential usage in radioimmunotherapy of glioma tumor in xenograft mice model. ［Methods］ Radioiodination of the Hsp90α-mAb was performed by the Chloramine-T method. 131I-Hsp90α-mAb were injected into the nude mice bearing human carcinoma via a tail vein. Biodistribution was performed at 6，12，24h after injection. Tumor volume was measured forty days continuous after the radioimmunotherapy.［Results］ The labeling efficiencies of Hsp90α-mAb reached 56% and the radiochemical purity exceeded 95% after purification. Biodistribution data showed that 131I-Hsp90α-mAb predominantly accumulated in the liver，spleen and tumor. The tumor volume after radioimmunotherapy was significant smaller than that in control group and unlabeled group. ［Conclusions］ The Hsp90α-mAb can be successfully labeled by Chloramine-T method with 131I. The Hsp90α-mAb can be uptake specifically by tumor tissue in mice with U87 tumor. It has a bright application prospect in radioimmunotherapy. |
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