谷传文,王 姗,郑金华.声动力疗法刺激肿瘤微环境中CD80与CD86表达的研究[J].肿瘤学杂志,2015,21(3):201-205. |
声动力疗法刺激肿瘤微环境中CD80与CD86表达的研究 |
Sonodynamic Therapy Facilating Expression of CD80 and CD86 in Tumor Microenvironment |
投稿时间:2014-10-30 |
DOI:10.11735/j.issn.1671-170X.2015.03.B008 |
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中文关键词: 声动力治疗 B16F10细胞 肿瘤微环境 |
英文关键词:sonodynamic therapy melanoma B16F10 cells tumor microenvironment |
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中文摘要: |
摘 要:[目的] 检测声动力治疗( sonodynamic therapy,SDT)对B16F10荷瘤鼠模型中肿瘤微环境T细胞共刺激分子CD80、CD86表达情况的影响。[方法] 以2.0W/cm2超声强度结合250mg/kg 5-氨基酮戊酸(5-ALA)治疗B16F10荷瘤鼠,测量不同实验组小鼠体重及肿瘤大小;免疫组织化学方法检测移植瘤微环境中CD80、CD86、IFN-γ和TNF-α表达情况。[结果] 与其他组相比,SDT组肿瘤体积缩小更加显著,体重变化无差异;SDT组CD80、CD86、IFN-γ和TNF-α均出现表达增高,且具有统计学意义。[结论] SDT能够加强T细胞共刺激分子的CD80、CD86的表达,促进抗肿瘤免疫的发生。 |
英文摘要: |
Abstract:[Purpose] To detect the variation of costimulatory molecules CD80 and CD86 in tumor microenvironment by sonodynamic therapy(SDT) in B16F10 tumor-bearing nude mice. [Methods] 2W/cm2 ultrasound intensity with 250mg/kg 5-Aminolevulinic acid (5-ALA)were used in B16F10 tumor-bearing nude mice,and the tumor volume and body weight of mice were measured in different groups respectively before and after treatment.CD80,CD86,IFN-γ and TNF-α in tumor microenvironment were detected by immunohistochemistry. [Results] Compared with other groups,variation of tumor volume in SDT group was statistically significant,while variation of body weight in SDT group was not significant;and expressions of CD80,CD86,IFN-γ and TNF-α in SDT group increased significantly.[Conclusion] SDT facilitates costimulatory molecules CD80 and CD86 secretion and promotes anti-tumor immunity. |
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