| 赵 璋,张 宁,龙 江.肝细胞癌基因组5q13.2与8p23.1位点的杂合性缺失分析[J].肿瘤学杂志,2015,21(1):34-38. |
| 肝细胞癌基因组5q13.2与8p23.1位点的杂合性缺失分析 |
| Loss of Heterozygosity in Two Distinct Regions，5q13.2 and 8p23.1 in Hepatocellular Carcinoma |
| 投稿时间：2014-03-11 |
| DOI：10.11735/j.issn.1671-170X.2015.01.B008 |
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| 中文关键词: 杂合性缺失 肝细胞癌 基因 |
| 英文关键词:loss of heterozygosity hepatocellular carcinoma gene |
| 基金项目:王宝恩肝纤维化基金（20100013）；北京市自然科学基金（7132058） |
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| 中文摘要: |
| 摘 要：［目的］ 分析肝细胞癌(HCC)中5q13.2和8p23.1位点的杂合性缺失（LOH）情况，以探索新的抑癌基因。［方法］ 应用聚合酶链反应—非变性聚丙烯酰胺凝胶电泳法对83例HCC患者基因组DNA中的5q13.2和8p23.1位点的LOH进行检测，并将其与临床参数进行关联分析。［结果］ 83例HCC患者基因组中，36.1%（30例）存在5q13.2位点LOH，在该位点存在OCLN、SMN2、SERF1A 、SMN1、NAIP和 GTF2H2基因；8p23.1的D8S503和D8S1130位点分别存在68.4%和61.3%的LOH。［结论］ HCC患者基因组中存在较高频率的5q13.2和8p23.1位点LOH，这些位点中可能存在与HCC发生密切相关的基因。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the frequency of loss of heterozygosity(LOH) at 5q13.2 and 8p23.1 in hepatocellular carcinoma (HCC)，and to explore possible tumor suppressor genes in the loci.［Methods］ The frequency of LOH at 5q13.2 and 8p23.1 were analyzed in 83 cases with HCC by polymerase chain reaction-polyacrylamide gel electrophoresis. Correlation analysis were performed between the frequency of LOH and clinicopathological characteristics of HCC.［Results］ Thirty of 83 HCC cases were indentified with LOH(36.1%) at 5q13.2，where a series of functional genes were harbored such as OCLN，SMN2，SERF1A，SMN1，NAIP and GTF2H2. The frequencies of LOH at D8S1130 and D8S503 in 8p23.1 were 61.29% and 68.4% respectively.［Conclusion］ In the genome of HCC，there are high frequency of LOH at 5q13.2 and 8p23.1，where critical genes associated with the development of HCC may harbor. |
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