| 余 昆,李云峰,杨之斌.GATA4基因甲基化在早期结直肠癌诊断中的作用[J].肿瘤学杂志,2015,21(1):30-33. |
| GATA4基因甲基化在早期结直肠癌诊断中的作用 |
| The Role of Methylation of GATA4 Gene on the Diagnosis of Colorectal Cancer Early Stage |
| 投稿时间：2014-09-24 |
| DOI：10.11735/j.issn.1671-170X.2015.01.B007 |
|
 |
| 中文关键词: GATA4基因 甲基化 结直肠肿瘤 粪便 |
| 英文关键词:GATA4 gene methylation colorectal neoplasms faeces |
| 基金项目: |
|
| 摘要点击次数: 1745 |
| 全文下载次数: 872 |
| 中文摘要: |
| 摘 要：［目的］ 检测早期结直肠癌（colorectal cancer，CRC）患者粪便中GATA4基因甲基化水平，探讨其临床价值，为CRC早期无创诊断探索新的途径。［方法］ 60例早期（Ⅰ+Ⅱ期）CRC患者、30例正常对照者粪便中提取DNA，采用巢式甲基化特异性PCR（nMSP）技术分析其GATA4基因甲基化状态。同时检测60例早期（Ⅰ+Ⅱ期）CRC患者血清CEA和粪便潜血。［结果］ CRC患者术前粪便标本中GATA4基因甲基化检测率显著高于正常对照组GATA4基因甲基化检测率和CRC患者粪便潜血检测率以及CEA检测率（76.7% vs 6.7%，P=0.000；vs 48.3%，P=0.001；vs 35.0%，P=0.000），而GATA4基因甲基化检出率与患者性别、年龄和肿瘤部位无明显相关（P>0.05）。［结论］ CRC早期患者粪便标本中GATA4基因甲基化检测率较高，优于粪便潜血实验和血清CEA，可作为早期无创筛查诊断结直肠癌的重要线索。 |
| 英文摘要: |
| Abstract：［Purpose］To detect methylation level of GATA4 gene in faeces from early colorectal cancer(CRC) patients，to investigate its clinical value and to explore a new non-invasive diagnostic process for early CRC. ［Methods］ Fecal DNA was collected from 60 cases with early CRC(stage Ⅰ+Ⅱ) and 30 normal controls，nested methylation specific PCR (nMSP) was used to analyze methylation status of GATA4 gene. Serum CEA and fecal occult blood were also detected in patients with early CRC(stage Ⅰ+Ⅱ). ［Results］ GATA4 gene methylation detection rate in early CRC patients was significantly higher than that in normal controls，and also was higher than detection rates of fecal occult blood and serum CEA respectively(76.7% vs. 6.7%，P=0.000；vs. 48.3%，P=0.001；vs. 35.0%，P=0.000)；and GATA4 gene methylation detection rate had no relation with gender，age and the location of tumor(P>0.05). ［Conclusion］ GATA4 gene methylation detection rate in faeces from early CRC patients is higher than detection rates of fecal occult blood and serum CEA，which provide an important way of non-invasive screening and diagnosis for early CRC. |
|
在线阅读
查看全文 查看/发表评论 下载PDF阅读器 |