| 江 慧,宋 纯,张桂荣.重组多肽EnpepNGR-27aa对人HUVEC细胞的抑制作用研究[J].肿瘤学杂志,2014,20(11):908-913. |
| 重组多肽EnpepNGR-27aa对人HUVEC细胞的抑制作用研究 |
| Study on Inhibition Effects of Recombinant Polypeptide EnpepNGR-27aa on Human HUVEC Cell |
| 投稿时间：2014-07-08 |
| DOI：10.11735/j.issn.1671-170X.2014.11.B008 |
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| 中文关键词: 血管内皮抑素 NGR模体 血管生成 |
| 英文关键词:endostatin NGR motif angiogenesis |
| 基金项目:辽宁省博士科研启动基金项目（20101059） |
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| 中文摘要: |
| 摘 要：［目的］ 研究含新生血管靶向基序NGR的血管内皮抑素（Endostatin）多肽对人HUVEC细胞的抑制作用。［方法］ 固相法合成Endostatin多肽Enpep-27aa和含NGR序列多肽EnpepNGR-27aa。MTT和BrdU法检测两种多肽对HUVEC细胞增殖的影响；MTT法分析两种多肽对HUVEC细胞黏附能力的影响；Transwell法检测两种多肽对HUVEC细胞迁移的影响。［结果］ 当干预浓度大于10nmol/L时，两种多肽均对HUVEC细胞增殖有明显的抑制作用，且EnpepNGR-27aa组抑制率高于Enpep-27aa组（P<0.05）；两种多肽均能明显降低HUVEC细胞的黏附能力，且EnpepNGR-27aa的效果更明显（P<0.05）；两种多肽均显著降低HUVEC细胞的迁移能力，但两组之间无显著差异（P=0.13）。［结论］ NGR基序的引入显著增强了Endostatin短肽对HUVEC细胞的增殖抑制能力，并降低了其黏附能力。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the inhibition effect of Endostatin polypeptide with neovascular targeting motif NGR on human HUVEC cell. ［Methods］ Endostatin polypeptides Enpep-27aa and EnpepNGR-27aa with NGR sequence were synthesized by solid state method. Proliferation effects of the two peptides on HUVEC cells were detected by MTT and BrdU assays. Cell adhesion effects of the two peptides on HUVEC cells were analyzed by MTT method. Transwell assay was used to detect the migration effects of the two peptides on HUVEC cells. ［Results］ Proliferation of HUVEC cells was inhibited by Enpep-27aa and EnpepNGR-27aa significantly. Inhibition rate induced by EnpepNGR-27aa was higher than that by Enpep-27aa when interventional concentration was greater than 10nmol/L(P<0.05). The adhesion ability of HUVEC cells was markedly reduced by the two peptides，and EnpepNGR-27aa was more effective than Enpep-27aa(P=0.003). Migration ability of HUVEC cells was significantly reduced by the two peptides，but no markedly difference was observed between EnpepNGR-27aa and Enpep-27aa groups(P=0.13). ［Conclusion］ NGR motif could significantly enhance the effects of proliferation and adhesion inhibition induced by Endostatin short peptide on HUVEC cells. |
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