| 王 剑,陈清勇,张晓敏.转染microRNA-330-5p对肺癌细胞侵袭和迁移能力的影响[J].肿瘤学杂志,2014,20(8):648-653. |
| 转染microRNA-330-5p对肺癌细胞侵袭和迁移能力的影响 |
| Impact of Transfection of MiR-330-5p on the Invasion and Metastasis of Lung Cancer Cells |
| 投稿时间：2014-04-30 |
| DOI：10.11735/j.issn.1671-170X.2014.08.B008 |
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| 中文关键词: miR-330-5p 哺乳动物雷帕霉素靶蛋白（mTOR） 肺肿瘤 侵袭 增殖 |
| 英文关键词:miR-330-5p mammalian target of rapamycin(mTOR) lung neoplasms infiltration proliferation |
| 基金项目:南京军区“334”高层次科技人才培养工程；浙江省科技计划项目（2013C33209）；杭州市医疗卫生科研项目（20130633B29） |
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| 中文摘要: |
| 摘 要：［目的］ 探讨miR-330-5p表达对肺癌细胞增殖、侵袭和迁移能力等生物学行为的影响，揭示miR-330-5p的作用机制。［方法］ 分析非小细胞肺癌（NSCLC）细胞系95C和95D的 miRNA表达谱芯片中miR-330-5p的表达情况并用靶标软件预测其靶基因；转染miR-330-5p类似物（mimics）至95D、转染miR-330-5p 抑制剂(inhibitor)至95C中，应用CCK-8法、transwell小室法检测miR-330-5p对肺癌细胞增殖、侵袭迁移等生物学行为的影响；应用Western blot法检测miR-330-5p表达对哺乳动物雷帕霉素靶蛋白（mTOR）表达水平的影响。［结果］ miRNA表达谱芯片显示miR-330-5p在95D中表达明显低于95C；靶标预测软件预测mTOR mRNA可能是miR-330-5p的靶基因；转染miR-330-5p mimics后95D细胞增殖能力显著降低，其24h侵袭穿膜细胞数明显低于对照组，mTOR蛋白表达明显下调(P<0.01)；而转染miR-330-5p inhibitor后95C细胞的增殖能力增强，其24h侵袭穿膜细胞数较对照组明显增加，mTOR蛋白表达上调(P<0.01)。［结论］ 提高miR-330-5p表达能够明显抑制肺癌细胞的增殖、侵袭与迁移能力，mTOR mRNA可能是其重要的靶基因。 |
| 英文摘要: |
| Abstract：［Purpose］ To investigate the effect of miR-330-5p on the proliferation，invasion and metastasis ability of lung cancer cells and to reveal the possible mechanisms of miR-330-5p.［Methods］ The expression level of miR-330-5p in non-small cell lung cancer (NSCLC) cell lines 95C and 95D was detected by miRNA expression profile chip technology，and the target genes were predicted by target genes prediction software. MiR-330-5p analogue(mimics) was transfected to high metastatic lung cancer cells (95D)，while miR-330-5p(inhibitor) was transfected to low metastatic lung cancer cells (95C). The proliferation，invasion and metastasis ability of lung cancer cells was detected by CCK-8 and Transwell Chambers method，and the expression level of mTOR protein was determined by Western blot method.［Results］ The results of miRNA expression profile chip showed that the expression level of miR-330-5p down-regulated in 95D and was significantly lower than that in 95C. mTOR mRNA may be a target gene of miR-330-5p predicting by target genes prediction software. After transfected with miR-330-5p mimics，the proliferation ability of 95D cells obviously decreased，the 24h number of cells with infiltration of membrane was significantly lower than that of the controls(P<0.01)，and the expression of mTOR protein down-regulated(P<0.01).While after transfected with miR-330-5p inhibitor，the proliferation ability of 95C obviously increased，the 24h number of cells with infiltration of membrane was significantly higher than that of the controls(P<0.01)，and the expression of mTOR protein up-regulated(P<0.01).［Conclusion］ Increasing the miR-330-5p expression level might significantly inhibit the proliferation，invasion and metastasis ability of lung cancer cells，and mTOR mRNA may be a target gene of miR-330-5p. |
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